PMID- 11978790
OWN - NLM
STAT- MEDLINE
DCOM- 20020827
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 27
DP  - 2002 Jul 5
TI  - Bi-directional regulation of UV-induced activation of p38 kinase and c-Jun 
      N-terminal kinase by G protein beta gamma-subunits.
PG  - 24197-203
AB  - Ultraviolet (UV) irradiation induces various cellular responses by activating 
      many UV-responsive enzymes including mitogen-activated protein kinases (MAPKs). 
      Various G protein-coupled receptor agonists also activate MAPKs, but it is not 
      known whether or not G proteins also mediate the UV-induced activation of MAPKs. 
      Therefore, this study was undertaken to determine whether the G protein 
      betagamma-subunit (Gbetagamma) mediates the UV-induced activation of p38 and JNK. 
      Gbetagamma overexpression in COS-1 cells amplified the UV-induced activation of 
      p38 but reduced JNK activation. The overexpression of the C-terminal region of 
      beta-adrenergic receptor kinase (betaARKct) decreased the UV-induced activation 
      of p38 but increased JNK activation. Gbeta(1)gamma(2) expression increased MKK3/6 
      phosphorylation with a concomitant decrease in MKK4 phosphorylation, which 
      contrasts with betaARKct expression. Gbeta(1)gamma(2) or betaARKct expression 
      resulted in corresponding changes in the transcriptional activity of CHOP and 
      c-Jun. Treatment with a p38 inhibitor, SB203580, or the expression of a 
      kinase-inactive p38 increased the UV-induced JNK activation. Expression of the 
      constitutively active MKK6 decreased the UV-induced JNK activation. In summary, 
      although the endogenous Gbetagamma was found to mediate about half of the 
      UV-induced activation of p38, it was found that exogenous Gbetagamma mediates the 
      bi-directional regulation of UV-induced p38 and JNK activation, and that this 
      bi-directional regulation results from the inhibition of JNK activation by the 
      p38 activated via Gbetagamma in the COS-1 cells.
FAU - Seo, MiRan
AU  - Seo M
AD  - Department of Biochemistry and Cancer Research Institute, Seoul National 
      University College of Medicine, Seoul 110-799, Korea.
FAU - Lee, Yun-Il
AU  - Lee YI
FAU - Cho, Chin-Ho
AU  - Cho CH
FAU - Bae, Chang-Dae
AU  - Bae CD
FAU - Kim, In-Hoo
AU  - Kim IH
FAU - Juhnn, Yong-Sung
AU  - Juhnn YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020426
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (G-protein Beta gamma)
RN  - 0 (GTP-Binding Protein beta Subunits)
RN  - 0 (GTP-Binding Protein gamma Subunits)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - Enzyme Activation
MH  - *GTP-Binding Protein beta Subunits
MH  - *GTP-Binding Protein gamma Subunits
MH  - Heterotrimeric GTP-Binding Proteins/chemistry/metabolism
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Kidney
MH  - Mitogen-Activated Protein Kinases/metabolism/*radiation effects
MH  - Recombinant Proteins/metabolism
MH  - *Ultraviolet Rays
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2002/04/30 10:00
MHDA- 2002/08/28 10:01
CRDT- 2002/04/30 10:00
PHST- 2002/04/30 10:00 [pubmed]
PHST- 2002/08/28 10:01 [medline]
PHST- 2002/04/30 10:00 [entrez]
AID - S0021-9258(19)66587-7 [pii]
AID - 10.1074/jbc.M201717200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Jul 5;277(27):24197-203. doi: 10.1074/jbc.M201717200. Epub 2002 
      Apr 26.