PMID- 11978978
OWN - NLM
STAT- MEDLINE
DCOM- 20020607
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 95
IP  - 1-2
DP  - 2001
TI  - Chromosome map of cosmid clones constructed with Chinese hamster genomic DNA.
PG  - 97-102
AB  - By fluorescence in situ hybridization (FISH), we constructed a chromosome map of 
      324 site-specific clones and 119 painting clones, which were isolated from a 
      cosmid library of Chinese hamster genomic DNA. The site-specific clones were 
      distributed among all chromosomes except chromosome 10 and the Y chromosome. On 
      the other hand, FISH signals of painting clones were mainly found in 
      heterochromatic regions. First, the various combinations of clones painting 
      centromeric regions suggest that the centromeric heterochromatin of Chinese 
      hamster chromosomes is extremely diverse in its repetitive sequence composition. 
      Second, the long arm of the X chromosome and the whole Y chromosome, both 
      heterochromatic regions, were simultaneously painted by many clones. The pattern 
      of co-localization indicated that a proximal segment of the long arm of the X 
      chromosome matches the complete long arm of the Y chromosome, whereas a distal 
      segment of the long arm of the X chromosome is comparable to the whole short arm 
      of the Y chromosome. Thus, these findings suggest that the corresponding segments 
      of the X and Y-chromosomes have common repeated DNA elements.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Ono, T
AU  - Ono T
AD  - Department of Genetics, Institute for Developmental Research, Aichi Human Service 
      Center, Kasugai, Japan.
FAU - Sonta, S
AU  - Sonta S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - Cloning, Molecular
MH  - Cosmids/*genetics
MH  - Cricetinae
MH  - Cricetulus/*genetics
MH  - *Genome
MH  - Genomic Library
MH  - Heterochromatin/genetics
MH  - Male
MH  - *Physical Chromosome Mapping
MH  - Sex Chromosomes/genetics
EDAT- 2002/04/30 10:00
MHDA- 2002/06/12 10:01
CRDT- 2002/04/30 10:00
PHST- 2002/04/30 10:00 [pubmed]
PHST- 2002/06/12 10:01 [medline]
PHST- 2002/04/30 10:00 [entrez]
AID - 57025 [pii]
AID - 10.1159/000057025 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2001;95(1-2):97-102. doi: 10.1159/000057025.