PMID- 11980978
OWN - NLM
STAT- MEDLINE
DCOM- 20020711
LR  - 20211203
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 5
DP  - 2002 May
TI  - Comparison of an automated ribotyping system to restriction endonuclease analysis 
      and pulsed-field gel electrophoresis for differentiating vancomycin-resistant 
      Enterococcus faecium isolates.
PG  - 1858-61
AB  - The RiboPrinter Microbial Characterization System was compared with pulsed-field 
      gel electrophoresis (PFGE), restriction endonuclease analysis (REA), and 
      epidemiological data for typing 45 vancomycin-resistant Enterococcus faecium 
      (VRE) isolates. In 21 clinically related isolates, 90 to 100% were similar by 
      PFGE and REA, but only 57% were similar by the RiboPrinter. In another eight 
      clinically related isolates, three isolates similar by PFGE and REA were all 
      unique by the RiboPrinter. In contrast, in 16 clinically unrelated isolates, the 
      predominant RiboPrinter ribotype represented 50% of the strains, while the 
      largest PFGE and REA clones represented less than 19% of the strains. These data 
      suggest that the RiboPrinter is not reliable for VRE investigation.
FAU - Price, Connie S
AU  - Price CS
AD  - Department of Pathology, Northwestern University and Northwestern Memorial 
      Hospital, Chicago, IL, USA. CSPrice@northwestern.edu
FAU - Huynh, Holly
AU  - Huynh H
FAU - Paule, Suzanne
AU  - Paule S
FAU - Hollis, Richard J
AU  - Hollis RJ
FAU - Noskin, Gary A
AU  - Noskin GA
FAU - Pfaller, Michael A
AU  - Pfaller MA
FAU - Peterson, Lance R
AU  - Peterson LR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Automation/methods
MH  - Electrophoresis, Gel, Pulsed-Field/methods
MH  - Enterococcus faecium/*classification/drug effects/genetics/isolation & 
      purification
MH  - Gram-Positive Bacterial Infections/*diagnosis
MH  - Hospital Units
MH  - Humans
MH  - Prohibitins
MH  - Restriction Mapping
MH  - Serotyping/methods
MH  - *Vancomycin Resistance
PMC - PMC130939
EDAT- 2002/05/01 10:00
MHDA- 2002/07/12 10:01
PMCR- 2002/05/01
CRDT- 2002/05/01 10:00
PHST- 2002/05/01 10:00 [pubmed]
PHST- 2002/07/12 10:01 [medline]
PHST- 2002/05/01 10:00 [entrez]
PHST- 2002/05/01 00:00 [pmc-release]
AID - 1432 [pii]
AID - 10.1128/JCM.40.5.1858-1861.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 May;40(5):1858-61. doi: 10.1128/JCM.40.5.1858-1861.2002.