PMID- 11982599
OWN - NLM
STAT- MEDLINE
DCOM- 20020611
LR  - 20190513
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 128
IP  - 1
DP  - 2002 Apr
TI  - Neonatal dendritic cells are intrinsically biased against Th-1 immune responses.
PG  - 118-23
AB  - Dendritic cells (DCs) were derived from human peripheral blood monocytes or cord 
      blood monocytes cultured in the presence of IL-4 and GM-CSF. Adult and cord DCs 
      were observed to have comparable immature phenotypes. However, the increase in 
      surface expression of HLA-DR and CD86 after addition of LPS was significantly 
      attenuated in cord DCs, with CD25 and CD83 expression also markedly reduced. Cord 
      DCs were also unable to produce IL-12p70, failed to down-regulate expression of 
      the chemokine receptor CCR5 and induced lower levels of IFN-gamma production from 
      allogeneic naive CD4+ T cells than their adult counterparts. In contrast, the 
      kinetics of the production of TNF-alpha and IL-10 in response to LPS stimulation 
      was comparable to adult DCs. The reduced ability of cord DCs to attain a fully 
      mature adult phenotype, and to activate naive CD4+ T cells to produce IFN-gamma, 
      suggests that they are intrinsically preprogrammed against the generation of Th-1 
      immune responses.
FAU - Langrish, C L
AU  - Langrish CL
AD  - Molecular Immunology Unit, University College London, UK.
FAU - Buddle, J C
AU  - Buddle JC
FAU - Thrasher, A J
AU  - Thrasher AJ
FAU - Goldblatt, D
AU  - Goldblatt D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Isoantigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - *Antigen Presentation
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/classification/drug effects/*immunology
MH  - Fetal Blood/cytology/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Infant, Newborn
MH  - Interleukin-10/biosynthesis
MH  - Isoantigens/immunology
MH  - Kinetics
MH  - Lipopolysaccharides/pharmacology
MH  - Th1 Cells/*immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
PMC - PMC1906380
EDAT- 2002/05/02 10:00
MHDA- 2002/06/12 10:01
PMCR- 2003/04/01
CRDT- 2002/05/02 10:00
PHST- 2002/05/02 10:00 [pubmed]
PHST- 2002/06/12 10:01 [medline]
PHST- 2002/05/02 10:00 [entrez]
PHST- 2003/04/01 00:00 [pmc-release]
AID - 1817 [pii]
AID - 10.1046/j.1365-2249.2002.01817.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2002 Apr;128(1):118-23. doi: 10.1046/j.1365-2249.2002.01817.x.