PMID- 11983447
OWN - NLM
STAT- MEDLINE
DCOM- 20021213
LR  - 20190817
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 36
IP  - 5
DP  - 2002 May
TI  - Inhibition of urokinase-type plasminogen activator delays expression of c-jun, 
      activated transforming growth factor beta 1, and matrix metalloproteinase 2 
      during post-hepatectomy liver regeneration in mice.
PG  - 637-44
AB  - BACKGROUND/AIMS: Although urokinase-type plasminogen activator (u-PA) is 
      suggested to initiate various factors in liver regeneration after hepatectomy, no 
      corroborative evidence has been reported. In the present study, we investigated 
      the effect of u-PA on liver regeneration after hepatectomy. METHODS: Mice were 
      placed into either a control group or a u-PA-inhibited group that received an in 
      vivo u-PA inhibitor, p-aminobenzamidine. After we had removed two-thirds of the 
      liver, we examined the expressions of c-jun mRNA and activated transforming 
      growth factor beta 1 (TGF-beta 1), matrix metalloproteinase-2 (MMP-2) activity, 
      and the level of hepatocyte and non-parenchymal cell proliferation in the two 
      groups. RESULTS: In the u-PA-inhibited group, the delays in c-jun mRNA 
      expression, hepatocyte proliferation, activated TGF-1 expression, and expression 
      of MMP-2 activity, were 2h, 1, 2, and 1 day, respectively, and the sinusoid 
      architecture was not restored by 10 days after hepatectomy. CONCLUSIONS: u-PA 
      inhibition delays the expression of c-jun mRNA, hepatocyte proliferation, and 
      restoration of the sinusoid architecture, suggesting that u-PA plays important 
      roles in liver regeneration after hepatectomy through control of a transcription 
      factor, c-jun expression.
FAU - Nomura, Kazuhiko
AU  - Nomura K
AD  - First Department of Surgery, Shinshu University School of Medicine, Asahi 3-1-1, 
      Nagano, Matsumoto 390-8621, Japan.
FAU - Miyagawa, Shinichi
AU  - Miyagawa S
FAU - Ayukawa, Koichi
AU  - Ayukawa K
FAU - Soeda, Junpei
AU  - Soeda J
FAU - Taniguchi, Shun-ichiro
AU  - Taniguchi S
FAU - Kawasaki, Seiji
AU  - Kawasaki S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Benzamidines)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 63GK9KT599 (4-aminobenzamidine)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Benzamidines/pharmacology
MH  - Cell Division/drug effects
MH  - Hepatectomy
MH  - Hepatocytes/cytology
MH  - Liver Regeneration/*physiology
MH  - Male
MH  - Matrix Metalloproteinase 2/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Proto-Oncogene Proteins c-jun/*genetics
MH  - RNA, Messenger/analysis
MH  - Serine Proteinase Inhibitors/pharmacology
MH  - Transforming Growth Factor beta/*genetics
MH  - Transforming Growth Factor beta1
MH  - Urokinase-Type Plasminogen Activator/*antagonists & inhibitors
EDAT- 2002/05/02 10:00
MHDA- 2002/12/17 04:00
CRDT- 2002/05/02 10:00
PHST- 2002/05/02 10:00 [pubmed]
PHST- 2002/12/17 04:00 [medline]
PHST- 2002/05/02 10:00 [entrez]
AID - S0168827802000247 [pii]
AID - 10.1016/s0168-8278(02)00024-7 [doi]
PST - ppublish
SO  - J Hepatol. 2002 May;36(5):637-44. doi: 10.1016/s0168-8278(02)00024-7.