PMID- 11983920 OWN - NLM STAT- MEDLINE DCOM- 20020611 LR - 20240314 IS - 0027-8424 (Print) IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 99 IP - 9 DP - 2002 Apr 30 TI - Neurotrophin secretion from hippocampal neurons evoked by long-term-potentiation-inducing electrical stimulation patterns. PG - 6386-91 AB - The neurotrophin (NT) brain-derived neurotrophic factor (BDNF) plays an essential role in the formation of long-term potentiation (LTP). Here, we address whether this modulation by BDNF requires its continuous presence, or whether a local increase in BDNF is necessary during a specific time period of LTP initiation. Using electrical field stimulation of primary cultures of hippocampal neurons, we demonstrate that short high-frequency bursts of stimuli that induce LTP evoke also an instantaneous secretion of BDNF. In contrast, stimuli at low frequencies, inducing long-term depression, do not enhance BDNF secretion, suggesting that BDNF is specifically present, and thus required, at the time of LTP induction. The field-stimulation-mediated BDNF secretion depends on the formation of action potentials and is induced by IP(3)-mediated Ca(2+) release from intracellular stores. Experiments, aimed at determining the sites of NT secretion that use NT6, showed similar patterns of surface labeling by field stimulation to those shown previously by high potassium. FAU - Gartner, Annette AU - Gartner A AD - Max Planck Institute of Neurobiology, Am Klopferspitz 18A, 82152 Martinsried, Germany. agaertner@ucl.ac.uk FAU - Staiger, Volker AU - Staiger V LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - E0399OZS9N (Cyclic AMP) RN - SY7Q814VUP (Calcium) SB - IM MH - Adenoviridae/genetics MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Calcium/*metabolism MH - Cells, Cultured MH - Cyclic AMP/metabolism MH - *Electric Stimulation MH - Electrophysiology MH - Enzyme-Linked Immunosorbent Assay MH - Hippocampus/*metabolism MH - *Long-Term Potentiation MH - Microscopy, Electron MH - Nerve Growth Factors/*metabolism MH - Neurons/*metabolism MH - Rats MH - Rats, Wistar MH - Time Factors PMC - PMC122958 EDAT- 2002/05/02 10:00 MHDA- 2002/06/12 10:01 PMCR- 2002/10/30 CRDT- 2002/05/02 10:00 PHST- 2002/05/02 10:00 [pubmed] PHST- 2002/06/12 10:01 [medline] PHST- 2002/05/02 10:00 [entrez] PHST- 2002/10/30 00:00 [pmc-release] AID - 99/9/6386 [pii] AID - 092129699 [pii] AID - 1296 [pii] AID - 10.1073/pnas.092129699 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6386-91. doi: 10.1073/pnas.092129699.