PMID- 11985670
OWN - NLM
STAT- MEDLINE
DCOM- 20020528
LR  - 20240322
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 105
IP  - 4
DP  - 2002 Apr
TI  - Local T-cell activation after segmental allergen challenge in the lungs of 
      allergic dogs.
PG  - 499-508
AB  - Dogs with immunoglobulin E (IgE) allergy for ragweed that are sensitized by 
      intrapulmonary exposure to ragweed can be used to study the pulmonary immune 
      response that is important in allergic asthma. Using this model, we tested the 
      hypothesis that T lymphocytes are activated locally in the airways shortly after 
      allergen exposure of the lungs. The airways of six allergic dogs and three 
      non-allergic dogs were exposed to ragweed by segmental allergen challenge (SAC). 
      T-cell subsets and T-cell activation in blood and bronchoalveolar lavage (BAL) 
      fluid were measured by flow cytometry before SAC and at 4, 24 and 72 hr 
      thereafter. SAC caused a statistically significant increase in the percentage of 
      major histocompatibility complex (MHC) class II-positive CD4 and CD8 T cells in 
      BAL fluid and a significant increase in the mean fluorescent activity of MHC 
      class II from 4 hr after SAC onward. This activation was significantly different 
      from that found in cells from lung lobes challenged with saline, or from lung 
      lobes in non-allergic dogs challenged with ragweed. The percentage of 
      CD45RA(bright) CD8 cells increased significantly in allergic dogs after both 
      ragweed and saline challenges. This was significantly higher than in non-allergic 
      dogs. We conclude that T-cell activation in the airways of dogs can be measured 
      after in vivo activation of the cells by measuring MHC class II and CD45RA 
      expression in BAL fluid T cells. Furthermore, in allergic dogs, T cells are 
      activated locally in the lungs within 4 hr after exposure to ragweed allergen. 
      These results suggest a role for T lymphocytes in the development of late-phase 
      allergic reactions in the airways.
FAU - Out, Theo A
AU  - Out TA
AD  - Academic Medical Centre B1-236 and CLB Sanquin Blood Supply Foundation, Clinical 
      Immunology Laboratory, PO Box 22700, 1100 DE Amsterdam, the Netherlands. 
      t.a.out@amc.uva.nl
FAU - Wang, Shan-Ze
AU  - Wang SZ
FAU - Rudolph, Karin
AU  - Rudolph K
FAU - Bice, David E
AU  - Bice DE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Dogs
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/analysis
MH  - Hypersensitivity, Immediate/*immunology
MH  - Leukocyte Common Antigens/analysis
MH  - Lung/*immunology
MH  - *Lymphocyte Activation
MH  - Models, Animal
MH  - T-Lymphocyte Subsets/*immunology
MH  - Time Factors
PMC - PMC1782676
EDAT- 2002/05/03 10:00
MHDA- 2002/05/29 10:01
PMCR- 2003/04/01
CRDT- 2002/05/03 10:00
PHST- 2002/05/03 10:00 [pubmed]
PHST- 2002/05/29 10:01 [medline]
PHST- 2002/05/03 10:00 [entrez]
PHST- 2003/04/01 00:00 [pmc-release]
AID - 1383 [pii]
AID - 10.1046/j.1365-2567.2002.01383.x [doi]
PST - ppublish
SO  - Immunology. 2002 Apr;105(4):499-508. doi: 10.1046/j.1365-2567.2002.01383.x.