PMID- 11986375
OWN - NLM
STAT- MEDLINE
DCOM- 20020916
LR  - 20190513
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 540
IP  - Pt 3
DP  - 2002 May 1
TI  - Developmental profiles of glutamate receptors and synaptic transmission at a 
      single synapse in the mouse auditory brainstem.
PG  - 861-73
AB  - Using whole-cell recordings from presynaptic terminals and postsynaptic principal 
      neurons in the mouse medial nucleus of the trapezoid body (MNTB), we have 
      characterized properties of the calyx of Held synapse during the first three 
      postnatal weeks. We observed that evoked excitatory postsynaptic currents (EPSCs) 
      mediated by NMDA receptors (NMDAR) increased until postnatal day 11/12 (P11/12) 
      after which they declined to very low or undetectable levels at P16. Meanwhile, 
      EPSCs mediated by AMPA receptors (AMPAR) showed an approximate three-fold 
      increase in amplitude. These changes were paralleled by NMDAR and AMPAR currents 
      evoked by exogenous NMDA and kainate to MNTB neurons except that whole-cell 
      kainate currents remained constant after P7/8 while AMPAR-EPSCs continued to 
      increase. We found that the decay time constant tau for NMDAR-EPSCs and 
      AMPAR-EPSCs declined by about 30 % and 70 %, respectively. Analyses of 
      NMDAR-EPSCs with subunit-specific pharmacological agents including ifenprodil, 
      N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN), zinc and Mg(2+) 
      revealed subtle developmental changes in subunit composition. As maturation 
      progressed, this synapse displayed a reduction in the number of presynaptic spike 
      failures and the extent of synaptic depression in response to trains of stimuli 
      (50-300 Hz) while the recovery rate from depression accelerated. These results 
      demonstrate profound changes in the size and kinetics of postsynaptic glutamate 
      receptors and in the spike-firing capability of presynaptic terminals at the 
      calyx of Held-MNTB synapse during early development. We suggest that these 
      concurrent presynaptic and postsynaptic adaptations represent important steps for 
      synapse consolidation and refinement and ultimately for the development of fast 
      high-fidelity transmission at this synapse.
FAU - Joshi, Indu
AU  - Joshi I
AD  - The Program for Brain and Behavioral Research & Division of Neurology, The 
      Hospital for Sick Children and Department of Physiology, University of Toronto, 
      555 University Avenue, Toronto, Ontario, Canada M5G 1X8.
FAU - Wang, Lu-Yang
AU  - Wang LY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Ethylenediamines)
RN  - 0 (Piperidines)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - I38ZP9992A (Magnesium)
RN  - J41CSQ7QDS (Zinc)
RN  - R8OE3P6O5S (ifenprodil)
RN  - R9PTU1U29I (N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Auditory Pathways/*growth & development
MH  - Brain Stem/*growth & development
MH  - Ethylenediamines/pharmacology
MH  - Excitatory Postsynaptic Potentials/physiology
MH  - Gene Expression Regulation, Developmental
MH  - Kinetics
MH  - Magnesium/pharmacology
MH  - Mice
MH  - Piperidines/pharmacology
MH  - Protein Subunits
MH  - Receptors, AMPA/genetics
MH  - Receptors, Glutamate/*genetics
MH  - Receptors, N-Methyl-D-Aspartate/drug effects/genetics
MH  - Synapses/*physiology
MH  - Synaptic Transmission/*physiology
MH  - Zinc/pharmacology
PMC - PMC2290274
EDAT- 2002/05/03 10:00
MHDA- 2002/09/17 10:01
PMCR- 2003/05/01
CRDT- 2002/05/03 10:00
PHST- 2002/05/03 10:00 [pubmed]
PHST- 2002/09/17 10:01 [medline]
PHST- 2002/05/03 10:00 [entrez]
PHST- 2003/05/01 00:00 [pmc-release]
AID - PHY_13506 [pii]
AID - 10.1113/jphysiol.2001.013506 [doi]
PST - ppublish
SO  - J Physiol. 2002 May 1;540(Pt 3):861-73. doi: 10.1113/jphysiol.2001.013506.