PMID- 11988017 OWN - NLM STAT- MEDLINE DCOM- 20020624 LR - 20220310 IS - 1044-7431 (Print) IS - 1044-7431 (Linking) VI - 19 IP - 4 DP - 2002 Apr TI - Anterograde transport and trophic actions of BDNF and NT-4/5 in the developing rat visual system. PG - 485-500 AB - During development the viability of immature neurons may depend upon retrograde, anterograde, or paracrine trophic support. Using (125)I-labeled peptides we show that there is substantial and rapid anterograde transport of brain-derived neurotrophic factor (BDNF) and, to a lesser extent, neurotrophin-4/5 (NT-4/5) to central visual target areas in the neonatal rat brain. Six hours after unilateral intraocular injection, all retinorecipient regions in the thalamus and midbrain are heavily labeled. Intraocular application of physiologically relevant doses of neurotrophin has a marked effect on cells in the developing superior colliculus (SC): 24 h postinjection of BDNF or NT-4/5, the number of pyknotic profiles in the contralateral superficial SC significantly decreases, while total cell numbers increase relative to ipsilateral SC. This increase is primarily associated with neurons. The data support the hypothesis that BDNF and NT-4/5 are anterograde survival factors for postsynaptic cells in the developing rat SC. CI - (c) 2002 Elsevier Science (USA). FAU - Spalding, Kirsty L AU - Spalding KL AD - School of Anatomy and Human Biology, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia. kirsty.spalding@cmb.ki.se FAU - Tan, Margaret M L AU - Tan MM FAU - Hendry, Ian A AU - Hendry IA FAU - Harvey, Alan R AU - Harvey AR LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Cell Neurosci JT - Molecular and cellular neurosciences JID - 9100095 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Coloring Agents) RN - 0 (Iodine Radioisotopes) RN - 0 (Nerve Growth Factors) RN - P658DCA9XD (neurotrophin 4) SB - IM MH - Animals MH - Animals, Newborn MH - Axonal Transport/*physiology MH - Brain/drug effects/*growth & development/metabolism MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Cell Count MH - Cell Differentiation/drug effects/*physiology MH - Cell Survival/drug effects/*physiology MH - Coloring Agents MH - Iodine Radioisotopes MH - Mice MH - Mice, Inbred BALB C MH - Nerve Growth Factors/*metabolism/pharmacology MH - Neuroglia/cytology/metabolism MH - Neurons/cytology/drug effects/*metabolism MH - Presynaptic Terminals/drug effects/metabolism/ultrastructure MH - Rats MH - Rats, Wistar MH - Retinal Ganglion Cells/cytology/drug effects/metabolism MH - Superior Colliculi/drug effects/growth & development/metabolism MH - Visual Pathways/drug effects/*growth & development/metabolism EDAT- 2002/05/04 10:00 MHDA- 2002/06/25 10:01 CRDT- 2002/05/04 10:00 PHST- 2002/05/04 10:00 [pubmed] PHST- 2002/06/25 10:01 [medline] PHST- 2002/05/04 10:00 [entrez] AID - S1044743101910978 [pii] AID - 10.1006/mcne.2001.1097 [doi] PST - ppublish SO - Mol Cell Neurosci. 2002 Apr;19(4):485-500. doi: 10.1006/mcne.2001.1097.