PMID- 11990231
OWN - NLM
STAT- MEDLINE
DCOM- 20020523
LR  - 20190922
IS  - 0277-0903 (Print)
IS  - 0277-0903 (Linking)
VI  - 39
IP  - 2
DP  - 2002 Apr
TI  - Increased interleukin-4 and decreased interferon gamma production in children 
      with asthma: function of atopy or asthma?
PG  - 159-65
AB  - Both atopy and asthma are claimed to be associated with a Th-2 cytokine pattern. 
      We sought to determine the contribution of atopy and asthma to the observed 
      Th-2/Th-1 imbalance in these conditions. Of 60 children aged 6-16 years that were 
      included in the study, 13 were nonatopic nonasthmatic, 15 atopic nonasthmatic, 14 
      nonatopic asthmatic, and 18 atopic asthmatic. Atopic children had positive skin 
      prick tests to grass pollens only. All children were studied after an 
      asymptomatic and drug-free period of at least three months. Total IgE was 
      measured in serum. Peripheral blood mononuclear cells were cultured and 
      stimulated in vitro with phytohemagglutinin and interferongamma (IFN-gamma) and 
      interleukin-4 (IL-4) measured in the supernatants. Total IgE was significantly 
      higher in atopic asthmatics compared to nonatopic asthmatics (p = 0.004), and 
      nonatopic nonasthmatics (p = 0.001), but was not different from atopic 
      nonasthmatics (p >0.05). On the other hand, IL-4 was significantly elevated in 
      atopic asthmatics and in nonatopic asthmatics compared to nonatopic nonasthmatics 
      (p = 0.037 and p = 0.009, respectively). Although atopic asthmatics had lower 
      IFN-gamma values than nonatopic asthmatics, the difference did not reach 
      statistical significance. No correlation was detected between any two parameters. 
      Our results suggest that both atopy and asthma contribute to the increased levels 
      of IL-4 and that, whereas nonatopic asthma is associated with increases in both 
      IL-4 and IFN-gamma release by mononuclear cells, only atopic asthma is 
      characterized by a Th-2 type cytokine dominance.
FAU - Akpinarli, Akgul
AU  - Akpinarli A
AD  - Department of Pediatrics, Hacettepe University School of Medicine, Ankara, 
      Turkey.
FAU - Guc, Dicle
AU  - Guc D
FAU - Kalayci, Omer
AU  - Kalayci O
FAU - Yigitbas, Emre
AU  - Yigitbas E
FAU - Ozon, Alev
AU  - Ozon A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Asthma/complications/*metabolism
MH  - Cells, Cultured
MH  - Child
MH  - Female
MH  - Humans
MH  - Hypersensitivity/complications/*metabolism
MH  - Immunoglobulin E/metabolism
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-4/*biosynthesis
MH  - Male
MH  - Monocytes/metabolism
MH  - Reference Values
EDAT- 2002/05/07 10:00
MHDA- 2002/05/25 10:01
CRDT- 2002/05/07 10:00
PHST- 2002/05/07 10:00 [pubmed]
PHST- 2002/05/25 10:01 [medline]
PHST- 2002/05/07 10:00 [entrez]
AID - 10.1081/jas-120002197 [doi]
PST - ppublish
SO  - J Asthma. 2002 Apr;39(2):159-65. doi: 10.1081/jas-120002197.