PMID- 11991983
OWN - NLM
STAT- MEDLINE
DCOM- 20020610
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 11
DP  - 2002 Jun
TI  - Characterizing and mapping porcine endogenous retroviruses in Westran pigs.
PG  - 5548-56
AB  - Since porcine endogenous retroviruses (PERVs) can infect cultured human cells, 
      they are a potential hazard to xenotransplantation. For this reason, endogenous 
      retroviruses from the Westran (Westmead Hospital transplantation) inbred line of 
      pigs were analyzed by using consensus primers for the type A and type B viruses 
      to amplify 1.8-kb envelope gene fragments. After preliminary analysis with 
      restriction enzymes KpnI and MboI, 31 clones were sequenced. Between types A and 
      B, five recombinant clones were identified. Fifty-five percent of clones (17 of 
      31) had premature stop codons within the envelope protein-encoding region. 
      Endogenous retroviruses in Westran pigs were physically mapped by fluorescence in 
      situ hybridization (FISH) using PERV-A and PERV-B envelope clones as probes to 
      identify at least 32 integration sites (19 PERV-A sites and 13 PERV-B sites). The 
      chromosomal sites of integration in the Westran strain are quite different from 
      those in the European Large White pig. The recombinant clones suggest that 
      defective PERVs could become infective through recombination and further that 
      PERVs might recombine with human endogenous retroviruses in xenotransplants.
FAU - Lee, Jun-Heon
AU  - Lee JH
AD  - Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of 
      Veterinary Science, The University of Sydney, Sydney, New South Wales 2006, 
      Australia.
FAU - Webb, Graham C
AU  - Webb GC
FAU - Allen, Richard D M
AU  - Allen RD
FAU - Moran, Chris
AU  - Moran C
LA  - eng
SI  - GENBANK/AF426916
SI  - GENBANK/AF426917
SI  - GENBANK/AF426918
SI  - GENBANK/AF426919
SI  - GENBANK/AF426920
SI  - GENBANK/AF426921
SI  - GENBANK/AF426922
SI  - GENBANK/AF426923
SI  - GENBANK/AF426924
SI  - GENBANK/AF426925
SI  - GENBANK/AF426926
SI  - GENBANK/AF426927
SI  - GENBANK/AF426928
SI  - GENBANK/AF426929
SI  - GENBANK/AF426930
SI  - GENBANK/AF426931
SI  - GENBANK/AF426932
SI  - GENBANK/AF426933
SI  - GENBANK/AF426934
SI  - GENBANK/AF426935
SI  - GENBANK/AF426936
SI  - GENBANK/AF426937
SI  - GENBANK/AF426938
SI  - GENBANK/AF426939
SI  - GENBANK/AF426940
SI  - GENBANK/AF426941
SI  - GENBANK/AF426942
SI  - GENBANK/AF426943
SI  - GENBANK/AF426944
SI  - GENBANK/AF426945
SI  - GENBANK/AF426946
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Codon, Terminator)
RN  - 0 (DNA, Viral)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GATC-specific type II deoxyribonucleases)
RN  - EC 3.1.21.4 (GGTACC-specific type II deoxyribonucleases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Codon, Terminator
MH  - DNA, Viral
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Endogenous Retroviruses/*genetics
MH  - Frameshift Mutation
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Swine/*virology
PMC - PMC137029
EDAT- 2002/05/07 10:00
MHDA- 2002/06/11 10:01
PMCR- 2002/06/01
CRDT- 2002/05/07 10:00
PHST- 2002/05/07 10:00 [pubmed]
PHST- 2002/06/11 10:01 [medline]
PHST- 2002/05/07 10:00 [entrez]
PHST- 2002/06/01 00:00 [pmc-release]
AID - 2133 [pii]
AID - 10.1128/jvi.76.11.5548-5556.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Jun;76(11):5548-56. doi: 10.1128/jvi.76.11.5548-5556.2002.