PMID- 11993643
OWN - NLM
STAT- MEDLINE
DCOM- 20020702
LR  - 20190826
IS  - 0910-6340 (Print)
IS  - 0910-6340 (Linking)
VI  - 17
IP  - 1
DP  - 2001 Jan
TI  - Application of capillary electrophoresis to the simultaneous determination of 
      betaines in plants.
PG  - 103-6
AB  - The simultaneous determination of betaines, the key compounds for osmotic 
      regulation in plants, was established by capillary electrophoresis (CE). After 
      four betaines, glycine betaine (GB), beta-alanine betaine (AB), proline betaine 
      (PB), and 2-hydroxyproline betaine (HPB), were esterified with p-bromophenacyl 
      bromide, the esters were electrophoresed in 100 mM sodium phosphate at pH 3.0. A 
      low-pH condition in CE and esterification gave an advantage of resolving each of 
      the ester peaks as well as those of the unreacted reagent and other components. 
      Furthermore, the addition of 4% polyethylene glycol (PEG) gave a better 
      resolution of 4 peaks, resulting in the separation of the overlapped peaks of PB 
      and AB. It was found from the standard addition method being applied to barley 
      leaves that the GB content in plants could be evaluated by using a calibration 
      curve of the GB standard solution. The extraction of GB from plant leaves was 
      also improved by adopting water as the extraction solvent instead of a mixture of 
      organic solvents. The present method was suitably applied to actual plant 
      specimens collected from a saline area of China.
FAU - Nishimura, N
AU  - Nishimura N
AD  - Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo, 
      Japan.
FAU - Zhang, J
AU  - Zhang J
FAU - Abo, M
AU  - Abo M
FAU - Okubo, A
AU  - Okubo A
FAU - Yamazaki, S
AU  - Yamazaki S
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Anal Sci
JT  - Analytical sciences : the international journal of the Japan Society for 
      Analytical Chemistry
JID - 8511078
RN  - 0 (Acetonitriles)
RN  - 0 (Esters)
RN  - 0 (Solvents)
RN  - 3SCV180C9W (Betaine)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Betaine/*analysis
MH  - Calibration
MH  - China
MH  - Electrophoresis, Capillary
MH  - Esters/chemistry
MH  - Plants/*chemistry
MH  - Reproducibility of Results
MH  - Solvents
EDAT- 2002/05/08 10:00
MHDA- 2002/07/03 10:01
CRDT- 2002/05/08 10:00
PHST- 2002/05/08 10:00 [pubmed]
PHST- 2002/07/03 10:01 [medline]
PHST- 2002/05/08 10:00 [entrez]
AID - 10.2116/analsci.17.103 [doi]
PST - ppublish
SO  - Anal Sci. 2001 Jan;17(1):103-6. doi: 10.2116/analsci.17.103.