PMID- 11993754
OWN - NLM
STAT- MEDLINE
DCOM- 20021029
LR  - 20191105
IS  - 1464-0325 (Print)
IS  - 1464-0325 (Linking)
VI  - 4
IP  - 2
DP  - 2002 Apr
TI  - A rapid and sensitive analytical method for the quantification of residues of 
      endosulfan in blood.
PG  - 190-3
AB  - A new sensitive analytical procedure has been developed for the determination of 
      residues of endosulfan in human blood samples. The method involves the extraction 
      of residues of endosulfan from blood samples by the addition of 60% sulfuric acid 
      at 10 degrees C, liquid/liquid partitioning by using hexane and acetone mixture 
      (9:1) and quantification by using GC-ECD. Residues of endosulfan in blood samples 
      were quantified as the sum of alpha-endosulfan, beta-endosulfan, endosulfan 
      sulfate and endosulfandiol. The influence of temperature during the extraction 
      has been studied. Recovery experiments were conducted over the concentration 
      range 1.0-50 ng ml(-1) and the relative standard deviation calculated. The method 
      was found to be sufficiently sensitive to quantify the residue of total 
      endosulfan up to the 1.0 ng ml(-1) level. The recovery was 92% with a calculated 
      relative standard deviation of 1.96%. Conversion of endosulfan to endosulfandiol 
      is found to be less than 0.5% under the defined conditions. The method was 
      applied to the analysis of residue contents of endosulfan and its metabolites in 
      blood samples collected from the exposed population. The data obtained has been 
      confirmed by GC-MS-EI in selective ion monitoring (SIM) mode.
FAU - Ramesh, Atmakuru
AU  - Ramesh A
AD  - Department of Pesticide Chemistry, Fredrick Institute of Plant Protection and 
      Toxicology (FIPPAT), Padappai, Chennai, Tamil Nadu, India. raamesh_a@hotmail.com
FAU - Ravi, Perumal Elumalai
AU  - Ravi PE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Environ Monit
JT  - Journal of environmental monitoring : JEM
JID - 100968688
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Insecticides)
RN  - 0 (Pesticide Residues)
RN  - OKA6A6ZD4K (Endosulfan)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Agriculture
MH  - Chromatography, Gas
MH  - Endosulfan/*blood
MH  - Female
MH  - Humans
MH  - *Hydrocarbons, Chlorinated
MH  - Insecticides/*blood
MH  - Male
MH  - Middle Aged
MH  - *Occupational Exposure
MH  - Pesticide Residues/*blood
MH  - Sensitivity and Specificity
MH  - Specimen Handling
MH  - Temperature
EDAT- 2002/05/08 10:00
MHDA- 2002/10/31 04:00
CRDT- 2002/05/08 10:00
PHST- 2002/05/08 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/05/08 10:00 [entrez]
AID - 10.1039/b110687m [doi]
PST - ppublish
SO  - J Environ Monit. 2002 Apr;4(2):190-3. doi: 10.1039/b110687m.