PMID- 11994790
OWN - NLM
STAT- MEDLINE
DCOM- 20020621
LR  - 20121115
IS  - 0090-4341 (Print)
IS  - 0090-4341 (Linking)
VI  - 42
IP  - 4
DP  - 2002 May
TI  - Effect of pyrene on hepatic cytochrome P450 1A (CYP1A) expression in Nile Tilapia 
      (Oreochromis niloticus).
PG  - 477-85
AB  - The effect of pyrene on the regulation of the gene expression of cytochrome 
      P4501A ( CYP1A) was studied in tilapia (Oreochromis niloticus), a tropical fish 
      of great ecological and economical importance. To evaluate CYP1A mRNA, tilapia 
      CYP1A cDNA was cloned, sequenced, and compared with those CYP1A reported 
      sequences in the GeneBank DNA database. The top seven matches corresponded to 
      CYP1A from other teleosts. Hepatic CYP1A mRNA levels showed a significant 
      increase at day 1 after pyrene injection (20 mg kg(-1) body weight [BW]), and 
      this CYP1A mRNA levels did not return to basal levels for up to 5 days. The 
      immunoblot analysis of CYP1A protein levels using polyclonal rabbit-anti-trout 
      antibodies in the liver of pyrene-treated (20 mg kg(-1) BW) tilapias showed a 
      1.9-fold increase at day 3 after injection. Ethoxyresorufin- O-deethylase (EROD) 
      activity increased 18-fold with respect to control fish at day 3 after injection. 
      CYP1A protein and EROD activity remained increased for 5 days after a single 
      pyrene IP administration. Similarly, the highest concentration of 1-hydroxypyrene 
      (1-OH pyrene) in bile was observed in fish sacrificed at day 3 after injection. 
      EROD activity and 1-OH pyrene concentration showed a statistically significant 
      correlation (r = 0.85) according to the Spearman test, suggesting the 
      participation of this cytochrome in the biotransformation of pyrene.
FAU - Zapata-Perez, O
AU  - Zapata-Perez O
AD  - Cinvestav-Merida, Depto de Recursos del Mar, Km. 6 antigua carretera a Progreso, 
      Col Cordemex. Apdo. Postal, 73, C.P. 97310, Merida, Yucatan, Mexico.
FAU - Gold-Bouchot, G
AU  - Gold-Bouchot G
FAU - Ortega, A
AU  - Ortega A
FAU - Lopez, T
AU  - Lopez T
FAU - Albores, A
AU  - Albores A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Environ Contam Toxicol
JT  - Archives of environmental contamination and toxicology
JID - 0357245
RN  - 0 (DNA, Complementary)
RN  - 0 (Pyrenes)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9E0T7WFW93 (pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cichlids/*metabolism
MH  - Cytochrome P-450 CYP1A1/biosynthesis/*genetics
MH  - DNA, Complementary/biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Induction
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Injections, Intraperitoneal
MH  - Microsomes, Liver/*drug effects/enzymology
MH  - Molecular Sequence Data
MH  - Pyrenes/*toxicity
MH  - Sequence Alignment
MH  - Water Pollutants, Chemical/*toxicity
EDAT- 2002/05/08 10:00
MHDA- 2002/06/22 10:01
CRDT- 2002/05/08 10:00
PHST- 2002/05/08 10:00 [pubmed]
PHST- 2002/06/22 10:01 [medline]
PHST- 2002/05/08 10:00 [entrez]
AID - 10.1007/s00244-001-0018-1 [doi]
PST - ppublish
SO  - Arch Environ Contam Toxicol. 2002 May;42(4):477-85. doi: 
      10.1007/s00244-001-0018-1.