PMID- 11995809
OWN - NLM
STAT- MEDLINE
DCOM- 20020904
LR  - 20190916
IS  - 0277-3740 (Print)
IS  - 0277-3740 (Linking)
VI  - 21
IP  - 2 Suppl 1
DP  - 2002 Mar
TI  - Respiratory syncytial virus may be a pathogen in allergic conjunctivitis.
PG  - S39-45
AB  - PURPOSE: The host response to allergens appears to be regulated by T helper type 
      2 cell patterns of local cytokine production. We hypothesized that respiratory 
      syncytial virus (RSV) infects the normal conjunctival epithelium and produces 
      interleukin (IL)-4, causing a local allergic reaction. METHODS: Giemsa staining, 
      immunocytochemical study, flow cytometric analysis, and reverse-transcriptase 
      polymerase chain reaction (RT-PCR) were performed on tear and cytology samples 
      from individuals with allergic and postoperative conjunctivitis. Histamine was 
      assayed by radioimmunoassay, and IL-4 by enzyme-linked immunosorbent assay. 
      RESULTS: The total number of cells collected by brush cytology did not differ 
      between allergic and postoperative conjunctivitis. The levels of IL-4 and 
      histamine in the specimens and mean IL-4 tear level were significantly increased 
      in patients with allergic conjunctivitis. Cytocentrifuge preparations contained 
      conjunctival epithelial cells with lymphocytes (a few CD3- and CD4-bearing 
      cells), mast cells, eosinophils, and higher human leukocyte antigen (HLA)-DR 
      expression in allergic patients. RT-PCR analysis showed that samples from 
      allergic conjunctivitis expressed increased transcripts of IL-4 and IL-13. A 
      higher percentage of RSV sequences were detected in allergic patient samples. 
      Immunocytochemical study and RT-PCR showed that epithelial cells were infected 
      with RSV. Average concentrations of IL-4 in culture supernatants were higher than 
      levels in uninfected cells. CONCLUSIONS: These results suggest that the clinical 
      features of human allergic conjunctivitis are associated with T helper type 2 
      cytokine expression. RSV in conjunctival epithelial cells may be an environmental 
      pathogen in allergic conjunctivitis.
FAU - Fujishima, Hiroshi
AU  - Fujishima H
AD  - Department of Ophthalmology, Tokyo Dental College, Sugano, Ichikawa, Japan. 
      fujishim@tdc.ac.jp
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
RN  - 0 (HLA-DR Antigens)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 207137-56-2 (Interleukin-4)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Conjunctivitis, Allergic/metabolism/*virology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/metabolism/virology
MH  - Eye Infections, Viral/metabolism/*virology
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/metabolism
MH  - Histamine/metabolism
MH  - Humans
MH  - Interleukin-4/metabolism
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/metabolism
MH  - RNA, Viral/analysis/isolation & purification
MH  - Radioimmunoassay
MH  - Respiratory Syncytial Virus Infections/metabolism/*virology
MH  - Respiratory Syncytial Virus, Human/genetics/isolation & 
      purification/*pathogenicity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tears/metabolism
EDAT- 2002/05/09 10:00
MHDA- 2002/09/06 10:01
CRDT- 2002/05/09 10:00
PHST- 2002/05/09 10:00 [pubmed]
PHST- 2002/09/06 10:01 [medline]
PHST- 2002/05/09 10:00 [entrez]
AID - 10.1097/00003226-200203001-00009 [doi]
PST - ppublish
SO  - Cornea. 2002 Mar;21(2 Suppl 1):S39-45. doi: 10.1097/00003226-200203001-00009.