PMID- 11995817
OWN - NLM
STAT- MEDLINE
DCOM- 20021030
LR  - 20190910
IS  - 0167-594X (Print)
IS  - 0167-594X (Linking)
VI  - 56
IP  - 2
DP  - 2002 Jan
TI  - Chromosome instability in malignant astrocytic tumors detected by fluorescence in 
      situ hybridization.
PG  - 159-65
AB  - Malignant tumors intrinsically manifest genetic instability, and consequently 
      genetic aberrations successively accumulate in tumor cells as the tumor 
      progresses. However, the relationship of genetic instability and biological 
      behavior still remains to be investigated in malignant tumors. In the present 
      investigation, the relationship between chromosomal instability and patient 
      prognosis was studied in 19 malignant gliomas. Chromosomal instability was 
      estimated by numerical variation in chromosomes 7, 10 and 17 which was measured 
      by fluorescence in situ hybridization (FISH), and DNA ploidy was determined by 
      laser scanning cytometry. The mean number of fractions was significantly higher 
      in cases with DNA aneuploidy than in those with DNA diploidy. The tendency toward 
      higher fractions in glioblastomas existed, although it did not reach statistical 
      significance. Kaplan-Meier survival rate analysis demonstrated significantly 
      lower survival rates in patients with higher fractions of chromosome 7 (>5) than 
      others. Our results suggest that DNA aneuploidy in malignant gliomas reflects an 
      underlying chromosomal instability, and that this instability is associated with 
      clinical behavior.
FAU - Nishizaki, Takafumi
AU  - Nishizaki T
AD  - Department of Neurosugery, Yamaguchi University School of Medicine, Japan. 
      nishi-ygc@umin.u-tokyo.ac.jp
FAU - Harada, Katsumi
AU  - Harada K
FAU - Kubota, Hisashi
AU  - Kubota H
FAU - Harada, Kei
AU  - Harada K
FAU - Furuya, Tomoko
AU  - Furuya T
FAU - Suzuki, Michiyasu
AU  - Suzuki M
FAU - Sasaki, Kohsuke
AU  - Sasaki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Astrocytoma/*genetics/mortality
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 10
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 7
MH  - DNA, Neoplasm/analysis
MH  - Diploidy
MH  - Female
MH  - Glioblastoma/*genetics/mortality
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prognosis
EDAT- 2002/05/09 10:00
MHDA- 2002/11/01 04:00
CRDT- 2002/05/09 10:00
PHST- 2002/05/09 10:00 [pubmed]
PHST- 2002/11/01 04:00 [medline]
PHST- 2002/05/09 10:00 [entrez]
AID - 10.1023/a:1014570720843 [doi]
PST - ppublish
SO  - J Neurooncol. 2002 Jan;56(2):159-65. doi: 10.1023/a:1014570720843.