PMID- 11996790
OWN - NLM
STAT- MEDLINE
DCOM- 20020515
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 134
IP  - 1
DP  - 2002 Apr 1
TI  - Fluorescence in situ hybridization improves cytogenetic results in the analysis 
      of hepatoblastoma.
PG  - 18-20
AB  - Hepatoblastoma (HB) is the most frequent malignant liver tumor in children. 
      Cytogenetic data indicate the presence of recurring trisomies of the chromosomes 
      2, 8, and 20, but more work is needed to clarify their incidence and prognostic 
      significance. Cytogenetic analysis is limited by the requirement of suitable 
      cells in metaphase. A different method that increases analysis sensitivity is 
      fluorescence in situ hybridization (FISH). We studied 20 cases of hepatoblastoma; 
      FISH analysis obtained results in 10 cases of HB with no informative karyotype. 
      In 5 of 10 of these cases at least one trisomic clone was detected, which always 
      coexisted with a population of diploid cells. These results confirm that trisomy 
      20 and/or 2 and 8 coexisting with diploid cells is a frequent finding in 
      hepatoblastoma and provide further support to the clonal evolution theory: 
      indeed, trisomy 20 was the most frequently detected abnormality, followed by 
      trisomy of chromosomes 2 and 8. In view of the high incidence of recurrent 
      trisomies, FISH analysis should be recommended in all the cases of HB with no 
      informative karyotype.
FAU - Sainati, L
AU  - Sainati L
AD  - Department of Pediatrics, University of Padua, Via Giustiniani 3, Padua, Italy. 
      laura.sainati@unipd.it
FAU - Leszl, A
AU  - Leszl A
FAU - Surace, C
AU  - Surace C
FAU - Perilongo, G
AU  - Perilongo G
FAU - Rocchi, M
AU  - Rocchi M
FAU - Basso, G
AU  - Basso G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Chromosomes, Human, Pair 20/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Cytogenetic Analysis
MH  - Female
MH  - Hepatoblastoma/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Liver Neoplasms/*genetics/pathology
MH  - Male
MH  - Trisomy
EDAT- 2002/05/09 10:00
MHDA- 2002/05/16 10:01
CRDT- 2002/05/09 10:00
PHST- 2002/05/09 10:00 [pubmed]
PHST- 2002/05/16 10:01 [medline]
PHST- 2002/05/09 10:00 [entrez]
AID - S0165460801005866 [pii]
AID - 10.1016/s0165-4608(01)00586-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Apr 1;134(1):18-20. doi: 
      10.1016/s0165-4608(01)00586-6.