PMID- 11996791
OWN - NLM
STAT- MEDLINE
DCOM- 20020515
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 134
IP  - 1
DP  - 2002 Apr 1
TI  - Significance of cytogenetic and fluorescence in situ hybridization analysis in 
      evaluating antichronic myeloid leukemia efficacy of different immune effector 
      cells.
PG  - 21-4
AB  - We report on the antileukemia effect of interleukin 2 (IL2) on different immune 
      cells from 22 patients with chronic myeloid leukemia (CML). Bone marrow cells 
      from these patients were first cultured in modified long-term bone marrow culture 
      medium for several days, then separately cultured with lymphokine activated 
      killer cells (LAK), cytokine-induced killer cells (CIK), and dendritic cell 
      cocultured CIK (DC-CIK) for another 1-2 days. They were then detected for 
      presence of the Philadelphia chromosome (Ph) by cytogenetic analysis and 
      fluorescence in situ hybridization (FISH). The percentage of 
      Ph-chromosome-positive cells in the bone marrow mononuclear cells after culturing 
      with CIK and DC-CIK was significantly lower than that after culturing with IL2 or 
      LAK. Our results demonstrate that cytogenetics and FISH are useful techniques for 
      the evaluation of the anti-CML effect of immune cells and that CIK or DC-CIK can 
      be appropriate candidates for adoptive immune cell therapy in vivo or for 
      leukemia cell purging ex vivo.
FAU - Tong, Chun Rong
AU  - Tong CR
AD  - Cellular Therapy Center, Institute of Hematology, People's Hospital, Peking 
      University, No. 11 South Street, Xi-Zhi-Men, 100044 Beijing, P. R. China. 
      phcet@public3.bta.net.com
FAU - Hong, Bo
AU  - Hong B
FAU - Qiu, Jing Ying
AU  - Qiu JY
FAU - Chen, Zhong
AU  - Chen Z
FAU - Lu, Dao Pei
AU  - Lu DP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow Cells/cytology/immunology/metabolism
MH  - Coculture Techniques
MH  - Cytogenetic Analysis
MH  - Dendritic Cells/cytology/drug effects/immunology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interleukin-2/pharmacology
MH  - Killer Cells, Lymphokine-Activated/cytology/drug effects/immunology
MH  - Killer Cells, Natural/cytology/drug effects/*immunology
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/immunology/pathology
MH  - Male
MH  - Philadelphia Chromosome
MH  - Tumor Cells, Cultured
EDAT- 2002/05/09 10:00
MHDA- 2002/05/16 10:01
CRDT- 2002/05/09 10:00
PHST- 2002/05/09 10:00 [pubmed]
PHST- 2002/05/16 10:01 [medline]
PHST- 2002/05/09 10:00 [entrez]
AID - S0165460801005891 [pii]
AID - 10.1016/s0165-4608(01)00589-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Apr 1;134(1):21-4. doi: 
      10.1016/s0165-4608(01)00589-1.