PMID- 11996799
OWN - NLM
STAT- MEDLINE
DCOM- 20020515
LR  - 20220317
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 134
IP  - 1
DP  - 2002 Apr 1
TI  - Characterization of a complex chromosome rearrangement involving 6q in a melanoma 
      cell line by chromosome microdissection.
PG  - 65-70
AB  - Deletion of 6q is one of the most frequent chromosomal alterations in human 
      malignant melanoma. Recently, we used chromosome painting probes of 6p and 6q to 
      study 21 melanoma cell lines. A reciprocal translocation between chromosomes 6q 
      and 17p was detected in one cell line (UACC-930). Upon further characterization 
      of the translocation marker using the micro fluorescence in situ hybridization 
      (FISH) technique, a complex rearrangement including an inversion of 6q and a 
      translocation between the inverted 6q and 17p, 
      [der(6)inv(6)(q16q27)t(6;17)(q26;p13)], was detected. A yeast artificial 
      chromosome (YAC) clone spanning the breakpoint at 6q16 was isolated by the FISH 
      screen. Loss of one or more copies of the YAC clone was also detected in 10 of 12 
      melanoma cell lines. This result implies that the YAC clone may contain a 
      putative tumor suppressor gene related to the pathogenesis of malignant melanoma. 
      Further characterizations of the breakpoint at 6q16 and molecular cloning 
      breakpoints at 6q27 and 17p13 are in progress.
FAU - Guan, Xin Yuan
AU  - Guan XY
AD  - Department of Clinical Oncology, The University of Hong Kong, Room 122, 
      Professorial Block, Pokfulam Road, Hong Kong, China. xyguan@hkucc.hku.hk
FAU - Zhang, Hong En
AU  - Zhang HE
FAU - Zhou, Hang
AU  - Zhou H
FAU - Sham, Jonathan S T
AU  - Sham JS
FAU - Fung, Jackie M-W
AU  - Fung JM
FAU - Trent, Jeffrey M
AU  - Trent JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Chromosome Breakage/genetics
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Melanoma/*genetics/pathology
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2002/05/09 10:00
MHDA- 2002/05/16 10:01
CRDT- 2002/05/09 10:00
PHST- 2002/05/09 10:00 [pubmed]
PHST- 2002/05/16 10:01 [medline]
PHST- 2002/05/09 10:00 [entrez]
AID - S0165460801006082 [pii]
AID - 10.1016/s0165-4608(01)00608-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Apr 1;134(1):65-70. doi: 
      10.1016/s0165-4608(01)00608-2.