PMID- 11996972
OWN - NLM
STAT- MEDLINE
DCOM- 20020710
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 502
IP  - 1-2
DP  - 2002 May 22
TI  - Use of yeast transformation by oligonucleotides to study DNA lesion bypass in 
      vivo.
PG  - 53-60
AB  - We have studied mutagenic specificities of DNA lesions in vivo in yeast CYC1 
      oligonucleotide transformation assay. We introduced two lesions into 
      oligonucleotides. One was a nucleoside analog, 
      3,4-dihydro-6H,8H-pyrimido[4,5-c][1,2]oxazin-7-one 2'-deoxyriboside (dP), which 
      is highly mutagenic to bacteria. It is supposed to be a miscoding, but otherwise 
      good template for DNA polymerases. The other lesion was the TT 
      pyrimidine(6-4)pyrimidone photoproduct, one of the typical UV lesions, which 
      blocks DNA replication. These oligonucleotides were used to transform yeast cyc1 
      mutants with ochre nonsense mutation to Cyc1+. As expected from its templating 
      properties in vitro, the transforming activity of dP-containing oligonucleotides 
      was similar to those of unmodified oligonucleotides. Results indicated that dP 
      may direct incorporation of guanine and adenine at a ratio of 1:20 or more in 
      vivo. An oligonucleotide containing the photoproduct showed the transforming 
      activity of as low as 3-5% of that of the corresponding unmodified 
      oligonucleotide. This bypass absolutely required REV1 gene. The sequence analysis 
      of the transformants has shown that the lesion was read as TT and TC at a ratio 
      of 3:7, indicating its high mutagenic potential.
FAU - Otsuka, Chie
AU  - Otsuka C
AD  - Gene Research Center, Okayama University, Tsushima, Okayama, Japan.
FAU - Kobayashi, Keita
AU  - Kobayashi K
FAU - Kawaguchi, Naho
AU  - Kawaguchi N
FAU - Kunitomi, Nozomu
AU  - Kunitomi N
FAU - Moriyama, Kei
AU  - Moriyama K
FAU - Hata, Yoshihiro
AU  - Hata Y
FAU - Iwai, Shigenori
AU  - Iwai S
FAU - Loakes, David
AU  - Loakes D
FAU - Noskov, Vladimir N
AU  - Noskov VN
FAU - Pavlov, Youri
AU  - Pavlov Y
FAU - Negishi, Kazuo
AU  - Negishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Fungal)
RN  - 0 (Mutagens)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Base Sequence
MH  - *DNA Damage
MH  - DNA Primers
MH  - DNA, Fungal/*genetics
MH  - Mutagens/*pharmacology
MH  - Oligonucleotides/*pharmacology
MH  - Saccharomyces cerevisiae/*genetics
EDAT- 2002/05/09 10:00
MHDA- 2002/07/12 10:01
CRDT- 2002/05/09 10:00
PHST- 2002/05/09 10:00 [pubmed]
PHST- 2002/07/12 10:01 [medline]
PHST- 2002/05/09 10:00 [entrez]
AID - S0027510702000234 [pii]
AID - 10.1016/s0027-5107(02)00023-4 [doi]
PST - ppublish
SO  - Mutat Res. 2002 May 22;502(1-2):53-60. doi: 10.1016/s0027-5107(02)00023-4.