PMID- 11999407
OWN - NLM
STAT- MEDLINE
DCOM- 20021114
LR  - 20061115
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 66
IP  - 2
DP  - 2002 Feb
TI  - Production of brain-derived neurotrophic factor in Escherichia coli by 
      coexpression of Dsb proteins.
PG  - 344-50
AB  - When brain-derived neurotrophic factor (BDNF) is produced in the Escherichia coli 
      periplasm, insoluble BDNF proteins with low biological activity and having 
      mismatched disulfide linkages are formed. The coexpression of cysteine 
      oxidoreductases (DsbA and DsbC) and membrane-bound enzymes (DsbB and DsbD), which 
      play an important role in the formation of disulfide bonds in the periplasm, was 
      investigated to improve the production of soluble and biologically active BDNF. 
      The expression levels of Dsb proteins changed when the growth medium and the Dsb 
      expression plasmids were changed, and the production rate of soluble BDNF was 
      almost proportional to the expression level of DsbC protein with disulfide 
      isomerase activity in the case of a low expression level of BDNF. The rate of 
      soluble BDNF production with coexpression of DsbABCD was as high as 35%. These 
      results show that coexpression of BDNF and Dsb proteins can effectively increase 
      the production of soluble and biologically active BDNF.
FAU - Hoshino, Kyoko
AU  - Hoshino K
AD  - Faculty of Life Sciences, Toyo University, Ora-gun, Gunma, Japan.
FAU - Eda, Akiko
AU  - Eda A
FAU - Kurokawa, Yoichi
AU  - Kurokawa Y
FAU - Shimizu, Norio
AU  - Shimizu N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media)
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Culture Media
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/*metabolism
MH  - Escherichia coli Proteins/*metabolism
EDAT- 2002/05/10 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/05/10 10:00
PHST- 2002/05/10 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/05/10 10:00 [entrez]
AID - 10.1271/bbb.66.344 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2002 Feb;66(2):344-50. doi: 10.1271/bbb.66.344.