PMID- 12000316
OWN - NLM
STAT- MEDLINE
DCOM- 20020718
LR  - 20191105
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 4
IP  - 3
DP  - 2002 Mar
TI  - Occurrence and activity of Archaea in aerated activated sludge wastewater 
      treatment plants.
PG  - 158-68
AB  - The occurrence, distribution and activity of archaeal populations within two 
      aerated, activated sludge wastewater treatment systems, one treating domestic 
      waste and the second treating mixed domestic and industrial wastewater, were 
      investigated by denaturing gradient gel electrophoresis (DGGE) analysis of 
      polymerase chain reaction (PCR)-amplified ribosomal RNA gene fragments and 
      process measurements. In the plant receiving mixed industrial and domestic waste 
      the archaeal populations found in the mixed liquor were very similar to those in 
      the influent sewage, though a small number of DGGE bands specific to the mixed 
      liquor were identified. In contrast, the activated sludge treating principally 
      domestic waste harboured distinct archaeal populations associated with the mixed 
      liquor that were not prevalent in the influent sewage. We deduce that the Archaea 
      in the plant treating mixed wastewater were derived principally from the 
      influent, whereas those in the plant treating solely domestic waste were actively 
      growing in the treatment plant. Archaeal 16S rRNA gene sequences related to the 
      Methanosarcinales, Methanomicrobiales and the Methanobacteriales were detected. 
      Methanogenesis was measured in activated sludge samples incubated under oxic and 
      anoxic conditions, demonstrating that the methanogens present in both activated 
      sludge plants were active only in anoxic incubations. The relatively low rates of 
      methanogenesis measured indicated that, although active, the methanogens play a 
      minor role in carbon turnover in activated sludge.
FAU - Gray, Neil D
AU  - Gray ND
AD  - Fossil Fuels and Environmental Geochemistry Postgraduate Institute (NRG), 
      University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
FAU - Miskin, Ian P
AU  - Miskin IP
FAU - Kornilova, Oksana
AU  - Kornilova O
FAU - Curtis, Thomas P
AU  - Curtis TP
FAU - Head, Ian M
AU  - Head IM
LA  - eng
SI  - GENBANK/AY055763
SI  - GENBANK/AY055764
SI  - GENBANK/AY055765
SI  - GENBANK/AY055766
SI  - GENBANK/AY055767
SI  - GENBANK/AY055768
SI  - GENBANK/AY055769
SI  - GENBANK/AY055770
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Archaeal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Archaea/genetics/*growth & development/metabolism
MH  - Base Sequence
MH  - DNA, Archaeal/chemistry/genetics
MH  - Methane/metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Sewage/*microbiology
MH  - United Kingdom
MH  - *Water Purification
EDAT- 2002/05/10 10:00
MHDA- 2002/07/19 10:01
CRDT- 2002/05/10 10:00
PHST- 2002/05/10 10:00 [pubmed]
PHST- 2002/07/19 10:01 [medline]
PHST- 2002/05/10 10:00 [entrez]
AID - 280 [pii]
AID - 10.1046/j.1462-2920.2002.00280.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2002 Mar;4(3):158-68. doi: 10.1046/j.1462-2920.2002.00280.x.