PMID- 12000711
OWN - NLM
STAT- MEDLINE
DCOM- 20020604
LR  - 20240315
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 160
IP  - 5
DP  - 2002 May
TI  - Gold-facilitated in situ hybridization: a bright-field autometallographic 
      alternative to fluorescence in situ hybridization for detection of Her-2/neu gene 
      amplification.
PG  - 1589-95
AB  - Fluorescence in situ hybridization (FISH) represents an excellent method for 
      profiling gene amplification in situ, but correlation with tissue morphology is 
      difficult because of dark-field visualization. Validation of a bright-field assay 
      for assessment of HER-2/neu gene amplification was investigated. 
      Streptavidin-Nanogold was used to generate bright-field gene copy signals using 
      GoldEnhance gold-based autometallography, catalyzed reported deposition, and a 
      biotin-labeled probe. One hundred cases of invasive breast carcinoma were 
      evaluated for which FISH gene copy results, and mRNA and oncoprotein gene 
      expression, were known. Autometallographic signals were qualitatively evaluable 
      without the use of oil immersion microscopy. Results correlated well with 
      indirect and direct label FISH. Autometallographic gold-based in situ 
      hybridization represents a promising bright-field assay for the assessment of 
      HER-2/neu gene amplification.
FAU - Tubbs, Raymond
AU  - Tubbs R
AD  - Department of Anatomic Pathology, The Cleveland Clinic Foundation, Cleveland, 
      Ohio 44195-5131, USA. tubbsr@ccf.org
FAU - Pettay, James
AU  - Pettay J
FAU - Skacel, Marek
AU  - Skacel M
FAU - Powell, Richard
AU  - Powell R
FAU - Stoler, Mark
AU  - Stoler M
FAU - Roche, Patrick
AU  - Roche P
FAU - Hainfeld, James
AU  - Hainfeld J
LA  - eng
GR  - R41 CA083618/CA/NCI NIH HHS/United States
GR  - R42 CA083618/CA/NCI NIH HHS/United States
GR  - 1R11 CA83618-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Gold Compounds)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/genetics/pathology
MH  - Carcinoma, Ductal, Breast/genetics/pathology
MH  - Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - *Gold Compounds
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Receptor, ErbB-2/*genetics
PMC - PMC1850888
EDAT- 2002/05/10 10:00
MHDA- 2002/06/05 10:01
PMCR- 2002/11/01
CRDT- 2002/05/10 10:00
PHST- 2002/05/10 10:00 [pubmed]
PHST- 2002/06/05 10:01 [medline]
PHST- 2002/05/10 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - S0002-9440(10)61106-6 [pii]
AID - 3122 [pii]
AID - 10.1016/S0002-9440(10)61106-6 [doi]
PST - ppublish
SO  - Am J Pathol. 2002 May;160(5):1589-95. doi: 10.1016/S0002-9440(10)61106-6.