PMID- 12001793
OWN - NLM
STAT- MEDLINE
DCOM- 20021113
LR  - 20161020
IS  - 1088-5412 (Print)
IS  - 1088-5412 (Linking)
VI  - 23
IP  - 2
DP  - 2002 Mar-Apr
TI  - Allergen cross-reactivity between proteins of the latex from Hevea brasiliensis, 
      seeds and pollen of Ricinus communis, and pollen of Mercurialis annua, members of 
      the Euphorbiaceae family.
PG  - 141-7
AB  - Allergen cross-reactions among three strongly sensitizing Euphorbiaceae species, 
      i.e., the rubber tree (Hevea brasiliensis), castor bean (Ricinus communis), and 
      the Mediterranean weed Mercurialis annua were studied in Finnish patients (n = 
      25) allergic to natural rubber latex (NRL), but with no known exposure to castor 
      bean or M. annua, and French patients allergic to castor bean (n = 26) or to M. 
      annua (n = 9), but not to NRL. In immunoglobulin E (IgE)-immunoblotting, 28% of 
      NRL-allergic patient sera recognized castor bean seed and 48% reacted to castor 
      bean pollen proteins. Likewise, 35% of the NRL-allergic patient sera bound to M. 
      annua pollen allergens. Nineteen percent of castor bean-allergic patients showed 
      IgE to NRL and 8% to M. annua proteins. Sera from patients allergic to M. annua 
      reacted in 44% to NRL, in 56% to castor bean seed, and in 78% to castor bean 
      pollen proteins. In immunoblotting, castor bean seed extract inhibited the 
      binding of NRL-reactive IgE to 20 kDa, 30 kDa of NRL, and 55 kDa of proteins; NRL 
      extract, in turn, inhibited the binding of castor bean-reactive IgE to 14, 21-22, 
      29, and 32-34 kDa of castor bean proteins. In ELISA inhibition, NRL extract 
      inhibited 33% of the binding of M. annua--reactive IgE of pooled sera to M. annua 
      pollen. In conclusion, allergen cross-reactivity in vitro was observed among 
      three botanically related Euphorbiaceae members, H. brasiliensis, R. communis, 
      and M. annua, but the molecular specificity of the observed cross-reactions as 
      well as their clinical significance remains to be elucidated. Allergen 
      cross-reactivity should be taken into account in diagnostic work.
FAU - Palosuo, Timo
AU  - Palosuo T
AD  - National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, 
      Finland.
FAU - Panzani, Raphael C
AU  - Panzani RC
FAU - Singh, Anand B
AU  - Singh AB
FAU - Ariano, Renato
AU  - Ariano R
FAU - Alenius, Harri
AU  - Alenius H
FAU - Turjanmaa, Kristiina
AU  - Turjanmaa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Allergy Asthma Proc
JT  - Allergy and asthma proceedings
JID - 9603640
RN  - 0 (Allergens)
RN  - 0 (Plant Proteins)
RN  - 9009-86-3 (Ricin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Allergens/immunology/*pharmacology
MH  - Child
MH  - Euphorbiaceae/*adverse effects/immunology
MH  - Female
MH  - Finland
MH  - Hevea/*adverse effects/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Latex Hypersensitivity/*etiology/*immunology
MH  - Male
MH  - Middle Aged
MH  - Plant Proteins/*adverse effects/immunology/*pharmacology
MH  - Pollen/*adverse effects/immunology
MH  - Ricin/*adverse effects/immunology/*pharmacology
MH  - Seeds/*adverse effects/immunology
EDAT- 2002/05/11 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/05/11 10:00
PHST- 2002/05/11 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/05/11 10:00 [entrez]
PST - ppublish
SO  - Allergy Asthma Proc. 2002 Mar-Apr;23(2):141-7.