PMID- 12002637
OWN - NLM
STAT- MEDLINE
DCOM- 20020923
LR  - 20061115
IS  - 0034-5288 (Print)
IS  - 0034-5288 (Linking)
VI  - 72
IP  - 1
DP  - 2002 Feb
TI  - Expression of tumour necrosis factor-alpha is associated with apoptosis in lungs 
      of pigs experimentally infected with porcine reproductive and respiratory 
      syndrome virus.
PG  - 45-9
AB  - Expression of tumour necrosis factor (TNF)-alpha and its association with 
      apoptosis was examined in lungs from pig experimentally inoculated with porcine 
      reproductive and respiratory syndrome virus (PRRSV). The TNF-alpha nucleic acid 
      was detected in PRRSV-infected alveolar macrophages by reverse 
      transcription-polymerase chain reaction (RT-PCR). The TNF-alpha antigen was 
      detected in alveolar macrophages by immunohistochemisty. DNA fragmentation in 
      apoptotic cells was detected by in situ terminal deoxynucleotidyl 
      transferase-mediated dUTP-biotin nick end labelling (TUNEL). A double-labelling 
      procedure using immunohistochemisty for the detection of PRRSV and the in situ 
      TUNEL assay for the detection of apoptosis demonstrated that a majority of 
      labelled cells were infected with PRRSV or apoptotic, but not both. Apoptotic 
      cells were more abundant than PRRSV-infected cells in all lungs examined. A 
      double-labelling procedure using immunohistochemisty for the detection of 
      TNF-alpha and the in situ TUNEL assay for the detection of apoptosis demonstrated 
      that a majority of labelled cells were apoptotic or for TNF-alpha, but not both. 
      These data suggested that TNF-alpha released from macrophages after infection by 
      PRRSV may induce apoptosis in uninfected bystander cells.
FAU - Choi, C
AU  - Choi C
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, Suwon, Republic of Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (RNA, Viral)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Gene Expression Regulation
MH  - In Situ Nick-End Labeling
MH  - Lung/*pathology/*virology
MH  - Macrophages, Alveolar/metabolism/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - RNA, Viral/genetics/metabolism
MH  - Swine/*virology
MH  - Swine Diseases/pathology/virology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics/*metabolism
EDAT- 2002/05/11 10:00
MHDA- 2002/09/24 06:00
CRDT- 2002/05/11 10:00
PHST- 2002/05/11 10:00 [pubmed]
PHST- 2002/09/24 06:00 [medline]
PHST- 2002/05/11 10:00 [entrez]
AID - S0034528801905193 [pii]
AID - 10.1053/rvsc.2001.0519 [doi]
PST - ppublish
SO  - Res Vet Sci. 2002 Feb;72(1):45-9. doi: 10.1053/rvsc.2001.0519.