PMID- 12003931
OWN - NLM
STAT- MEDLINE
DCOM- 20020607
LR  - 20210526
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 184
IP  - 11
DP  - 2002 Jun
TI  - Identification and physical characterization of the HbpR binding sites of the 
      hbpC and hbpD promoters.
PG  - 2914-24
AB  - Pseudomonas azelaica HBP1 can use 2-hydroxybiphenyl (2-HBP) and 
      2,2'-dihydroxybiphenyl as sole carbon and energy sources by means of the hbp 
      regulon. This regulon is composed of three genes, hbpCA and hbpD, coding for 
      enzymes of a meta-cleavage pathway and the hbpR gene, which codes for a 
      XylR/DmpR-type transcription regulator. It was previously shown that HbpR 
      activates transcription from two sigma(54)-dependent promoters, P(hbpC) and 
      P(hbpD), in the presence of 2-HBP. In this study, by using gel mobility shift 
      assays with a purified fusion protein containing calmodulin binding protein (CBP) 
      and HbpR, we detected two binding regions for HbpR in P(hbpC) and one binding 
      region in P(hbpD). DNase I footprints of the proximal binding region of P(hbpC) 
      and of the binding region in P(hbpD) showed that CBP-HbpR protected a region 
      composed of two inverted repeat sequences which were homologous to the binding 
      sites identified for XylR. Unlike the situation in the XylR/P(u) system, we 
      observed simultaneous binding of CBP-HbpR on the two upstream activating 
      sequences (UASs). Fragments with only one UAS did not show an interaction with 
      HbpR, indicating that both pairs of UASs are needed for HbpR binding. The 
      addition of both ATP and 2-HBP increased the DNA binding affinity of HbpR. These 
      results showed for the first time that, for regulators of the XylR/DmpR type, the 
      effector positively affects the recruitment of the regulatory protein on the 
      enhancer DNA.
FAU - Tropel, David
AU  - Tropel D
AD  - Process of Environmental Microbiology and Molecular Ecotoxicology, Swiss Federal 
      Institute for Environmental Science and Technology (EAWAG), CH-8600 Dubendorf, 
      Switzerland.
FAU - van der Meer, Jan Roelof
AU  - van der Meer JR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Calmodulin-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HBP2 protein)
RN  - 0 (HbpR protein, Pseudomonas azelaica)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (XylR protein, Pseudomonas)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Bacterial Proteins/*metabolism
MH  - Base Sequence
MH  - Binding Sites
MH  - Calmodulin-Binding Proteins/metabolism
MH  - DNA Footprinting
MH  - DNA-Binding Proteins/*metabolism
MH  - Deoxyribonuclease I
MH  - Electrophoretic Mobility Shift Assay
MH  - Enhancer Elements, Genetic
MH  - Escherichia coli/metabolism
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Pseudomonas/*genetics
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Trans-Activators/pharmacology
MH  - Transcription Factors/*metabolism
PMC - PMC135056
EDAT- 2002/05/11 10:00
MHDA- 2002/06/12 10:01
PMCR- 2002/06/01
CRDT- 2002/05/11 10:00
PHST- 2002/05/11 10:00 [pubmed]
PHST- 2002/06/12 10:01 [medline]
PHST- 2002/05/11 10:00 [entrez]
PHST- 2002/06/01 00:00 [pmc-release]
AID - 1341 [pii]
AID - 10.1128/JB.184.11.2914-2924.2002 [doi]
PST - ppublish
SO  - J Bacteriol. 2002 Jun;184(11):2914-24. doi: 10.1128/JB.184.11.2914-2924.2002.