PMID- 12007891
OWN - NLM
STAT- MEDLINE
DCOM- 20020710
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 86
IP  - 3-4
DP  - 2002 Jul
TI  - Development of a baculovirus expression system for soluble porcine tumor necrosis 
      factor receptor type I and soluble porcine tumor necrosis factor receptor type 
      I-IgG fusion protein.
PG  - 251-4
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a key mediator of inflammatory 
      responses and gram-negative bacterial sepsis, but the role that it plays during 
      Salmonella enterica species bacterial infections in swine has not yet been 
      elucidated. To facilitate studies on the role of TNF-alpha on the pathology 
      associated with Salmonella infections in pigs, recombinant soluble porcine TNF 
      receptor type I (rspTNF-RI) and soluble TNF receptor type I fused to the Fc 
      region of porcine IgG1 (rspTNF-RI-IgG) were expressed in insect cells using a 
      baculovirus expression system. The proteins were secreted into the cell culture 
      media and purified by anti-soluble porcine TNF-RI antibody and protein G affinity 
      chromatography, respectively. The yield of protein using this method was 
      approximately 1.5mg rspTNF-RI and 4mg rspTNF-RI-IgG/L of cell culture medium. In 
      in vitro assays, rspTNF-RI-IgG was approximately 10-fold (0.97 vs. 10.00pmol/ml) 
      more effective than rspTNF-RI at completely inhibiting the cytotoxic activity of 
      500U of recombinant porcine TNF-alpha on 3 x 10(4) WEHI 164 murine fibrosarcoma, 
      clone 13, cells. Compared to previously described methods, this method yields 
      significantly more biologically active rspTNF-RI.
FAU - Mwangi, Simon Musyoka
AU  - Mwangi SM
AD  - National Animal Disease Center, ARS, USDA, 2300 Dayton Avenue, Ames, IA 50010, 
      USA.
FAU - Stabel, Thomas J
AU  - Stabel TJ
FAU - Kehrli, Marcus E Jr
AU  - Kehrli ME Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*biosynthesis/genetics/isolation & purification
MH  - Baculoviridae/*genetics
MH  - Immunoglobulin G/*biosynthesis/genetics/isolation & purification
MH  - Mice
MH  - Receptors, Tumor Necrosis Factor/*biosynthesis/genetics/isolation & purification
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Recombinant Fusion Proteins/*biosynthesis/genetics/isolation & purification
MH  - Spodoptera/metabolism/virology
MH  - Swine
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/immunology
EDAT- 2002/05/15 10:00
MHDA- 2002/07/12 10:01
CRDT- 2002/05/15 10:00
PHST- 2002/05/15 10:00 [pubmed]
PHST- 2002/07/12 10:01 [medline]
PHST- 2002/05/15 10:00 [entrez]
AID - S016524270200034X [pii]
AID - 10.1016/s0165-2427(02)00034-x [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2002 Jul;86(3-4):251-4. doi: 
      10.1016/s0165-2427(02)00034-x.