PMID- 12008958
OWN - NLM
STAT- MEDLINE
DCOM- 20030102
LR  - 20220410
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 87
IP  - 4
DP  - 2002 Apr
TI  - Brain-derived neurotrophic factor is stored in human platelets and released by 
      agonist stimulation.
PG  - 728-34
AB  - Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, 
      plays critical roles in the survival, growth, and maintenance of brain and 
      peripheral neurons. We report the presence of BDNF protein in human platelets and 
      its release upon agonist stimulation. The BDNF content of washed platelets varied 
      widely, from 3.5 to 67 ng/4 x 10(8) platelets, averaging 25.2 +/- 21.2 ng/4 x 
      10(8) platelets (mean+/-SD). The BDNF concentration in platelet-poor plasma was 
      low (1.7+/-1.7 ng/ml, n = 11). Thrombin, collagen, the Ca++ ionophore A23187, and 
      shear stress each induced a rapid release of BDNF from platelets. Up to only half 
      of platelet BDNF was secreted upon agonist stimulation, suggesting that platelets 
      may have a non-releasable pool of BDNF, or that the released BDNF binds to a 
      recognition site on the platelet surface and is internalized, as occurs with 
      serotonin. However, the cognate BDNF receptor, TrkB, was not detected in 
      platelets. Nevertheless, the ability of BDNF to bind washed platelets was shown 
      by FACS analysis confocal microscopy and by the binding and apparent 
      internalization of [125I]-BDNF by platelets. A very high affinity site (Kd = 130 
      x 10(-15) M, approximately 80 sites/platelet) and a moderately high affinity site 
      (Kd = 20 nM, approximately 3750 sites/platelet) were identified. The BDNF content 
      in two megakaryocytic cell lines, DAMI and Meg-01, was only 0.1% of the content 
      measured in platelets. No BDNF mRNA was detected by Northern blotting in these 
      cell lines or in platelets. The pituitary gland was also ruled out as a source 
      for platelet BDNF, since the BDNF content of rat platelets did not decrease 2 
      weeks after hypophysectomy. Thus, platelet BDNF is not acquired from the 
      megakaryocyte or pituitary gland, but is probably acquired from other sources via 
      the blood circulation. Platelets appear to bind, store and release BDNF upon 
      activation at the site of traumatic injury to facilitate the repair of peripheral 
      nerves or other tissues that contain TrkB.
FAU - Fujimura, Hironobu
AU  - Fujimura H
AD  - Otsuka Maryland Research Institute, LLC, Rockville 20850, USA.
FAU - Altar, C Anthony
AU  - Altar CA
FAU - Chen, Ruoyan
AU  - Chen R
FAU - Nakamura, Takashi
AU  - Nakamura T
FAU - Nakahashi, Takeshi
AU  - Nakahashi T
FAU - Kambayashi, Jun-ichi
AU  - Kambayashi J
FAU - Sun, Bing
AU  - Sun B
FAU - Tandon, Narendra N
AU  - Tandon NN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ionophores)
RN  - 0 (RNA, Messenger)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.4.21.5 (Thrombin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blood Platelets/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/*blood/genetics/metabolism
MH  - Calcimycin/pharmacology
MH  - Calcium/physiology
MH  - Cell Compartmentation
MH  - Collagen/pharmacology
MH  - Humans
MH  - Hypophysectomy
MH  - Ionophores/pharmacology
MH  - Male
MH  - Megakaryocytes/metabolism
MH  - Pituitary Gland/metabolism
MH  - Platelet Activation/*drug effects/physiology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptor, trkB/metabolism
MH  - Stress, Mechanical
MH  - Thrombin/pharmacology
EDAT- 2002/05/15 10:00
MHDA- 2003/01/03 04:00
CRDT- 2002/05/15 10:00
PHST- 2002/05/15 10:00 [pubmed]
PHST- 2003/01/03 04:00 [medline]
PHST- 2002/05/15 10:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 2002 Apr;87(4):728-34.