PMID- 12009026
OWN - NLM
STAT- MEDLINE
DCOM- 20021104
LR  - 20231020
IS  - 0253-6269 (Print)
IS  - 0253-6269 (Linking)
VI  - 25
IP  - 2
DP  - 2002 Apr
TI  - DNA dynamics: a fluorescence resonance energy transfer study using a 
      long-lifetime metal-ligand complex.
PG  - 143-50
AB  - Fluorescent probes bound to DNA typically display nanosecond decay times and 
      reveal only nanosecond motions. We extend the time range of measurable DNA 
      dynamics using [Ru(bpy)2(dppz)]2+ (bpy=2,2'-bipyridine, 
      dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuBD) which displays a mean lifetime near 
      90 ns. To test the usefulness of RuBD as a probe for diffusive processes in calf 
      thymus DNA, we compared the efficiencies of fluorescence resonance energy 
      transfer (FRET) using three donors which display lifetimes near 5 ns for acridine 
      orange (AO), 22 ns for ethidium bromide (EB) and 92 ns for RuBD, with nile blue 
      (NB) as the acceptor. The Forster distances for AO-NB, EB-NB and RuBD-NB 
      donor-acceptor pairs were 42.3, 52.3, and 30.6 A, respectively. All three donors 
      showed dramatic decreases in fluorescence intensities and more rapid intensity 
      decays with increasing NB concentrations. The intensity decays of AO and EB in 
      the presence of varying concentrations of NB were satisfactorily described by the 
      one-dimensional FRET model without diffusion (Blumen and Manz, 1979). In the case 
      of the long-lifetime donor RuBD, the experimental phase and modulation somewhat 
      deviated from the recovered values computed from this model. The recovered NB 
      concentrations and FRET efficiencies from the model were slightly larger than the 
      expected values, however, the recovered and expected values did not show a 
      significant difference. Thus, it is suggested that the lifetime of RuBD is too 
      short to measure diffusive processes in calf thymus DNA.
FAU - Kang, Jung Sook
AU  - Kang JS
AD  - Department of Oral Biochemistry and Molecular Biology, College of Dentistry and 
      Research Institute for Oral Biotechnology, Pusan National University, Korea. 
      jsokang@pusan.ac.kr
FAU - Lakowicz, Joseph R
AU  - Lakowicz JR
FAU - Piszczek, Grzegorz
AU  - Piszczek G
LA  - eng
GR  - P41 RR008119/RR/NCRR NIH HHS/United States
GR  - RR 08119/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Indicators and Reagents)
RN  - 0 (Metals)
RN  - 9007-49-2 (DNA)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - DNA/*chemistry
MH  - Diffusion
MH  - Energy Transfer
MH  - Ethidium/chemistry
MH  - Indicators and Reagents
MH  - Metals/chemistry
MH  - Spectrometry, Fluorescence
PMC - PMC6904224
MID - NIHMS1061642
EDAT- 2002/05/15 10:00
MHDA- 2002/11/26 04:00
PMCR- 2019/12/10
CRDT- 2002/05/15 10:00
PHST- 2002/05/15 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/05/15 10:00 [entrez]
PHST- 2019/12/10 00:00 [pmc-release]
AID - 10.1007/BF02976554 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2002 Apr;25(2):143-50. doi: 10.1007/BF02976554.