PMID- 12011256
OWN - NLM
STAT- MEDLINE
DCOM- 20020723
LR  - 20171116
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 15
IP  - 5
DP  - 2002 May
TI  - A comparative analysis of FISH, RT-PCR, PCR, and immunohistochemistry for the 
      diagnosis of mantle cell lymphomas.
PG  - 517-25
AB  - Mantle cell lymphoma (MCL) diagnosis first relies on morphology and phenotype 
      that may overlap with other B-cell lymphomas. Therefore, the demonstration of 
      t(11;14)(q13;q32), the cytogenetic hallmark of MCL, is considered of diagnostic 
      value. By studying a series of 35 MCL with characteristic morphology and 
      phenotype (CD5+, CD10-, CD20+, CD23-), we have evaluated the applicability and 
      the sensitivity of interphase fluorescence in situ hybridization (FISH) for 
      t(11;14) detection and other techniques: (1) polymerase chain reaction (PCR) for 
      amplification of t(11;14) genomic breakpoint, (2) competitive RT-PCR for the 
      detection of cyclin D1 transcripts overexpression, and (3) immunohistochemistry 
      (IHC) for cyclin D1 protein detection. Tissues from different origins were 
      analyzed: lymph nodes (n = 24), spleen (n = 3), digestive biopsy (n = 3), tonsils 
      (n = 3), and skin (n = 2). Interphase FISH was performed either on touch 
      preparations (n = 11) and frozen (n = 9) or paraffin sections (n = 15). FISH 
      analysis detected t(11;14) in 34/35 cases (97%) and demonstrated a recurrent 
      CCND1 amplification in t(11;14)+ nuclei of the three blastoid MCL variants of our 
      series. Genomic PCR analysis, hampered by the scattering of 11q13 breakpoints, 
      was positive in only 13/35 cases (37%). RT-PCR analysis was applicable on 
      nonepithelial tissues (27/35) and showed cyclin D1 transcript overexpression in 
      all tested cases (27/35). IHC for cyclin D1 protein was performed either on 
      frozen (n = 12) or on paraffin sections (n = 23), and its sensitivity was higher 
      on paraffin sections (91%) than on frozen sections (25%). A cyclin D1 protein 
      immunoreactivity was observed in 24/35 cases (69%). Our study emphasizes on the 
      use of FISH analysis for the direct detection of t(11;14) because its 
      applicability and sensitivity largely exceeded those of other techniques. It may 
      also provide some informations on secondary cytogenetic changes of potential 
      clinical relevance.
FAU - Belaud-Rotureau, Marc-Antoine
AU  - Belaud-Rotureau MA
AD  - Department of Histology and Molecular Pathology, Victor Segalen University, 
      Bordeaux, France. jp.merlio@histo.u-bordeaux2.fr
FAU - Parrens, Marie
AU  - Parrens M
FAU - Dubus, Pierre
AU  - Dubus P
FAU - Garroste, Jean-Christophe
AU  - Garroste JC
FAU - de Mascarel, Antoine
AU  - de Mascarel A
FAU - Merlio, Jean-Philippe
AU  - Merlio JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Antigens, CD20)
RN  - 0 (CD5 Antigens)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Antigens, CD20/analysis
MH  - Blotting, Southern
MH  - CD5 Antigens/analysis
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Cyclin D1/analysis/genetics
MH  - DNA, Neoplasm/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Mantle-Cell/genetics/metabolism/*pathology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Translocation, Genetic
EDAT- 2002/05/16 10:00
MHDA- 2002/07/24 10:01
CRDT- 2002/05/16 10:00
PHST- 2002/05/16 10:00 [pubmed]
PHST- 2002/07/24 10:01 [medline]
PHST- 2002/05/16 10:00 [entrez]
AID - 10.1038/modpathol.3880556 [doi]
PST - ppublish
SO  - Mod Pathol. 2002 May;15(5):517-25. doi: 10.1038/modpathol.3880556.