PMID- 12014684
OWN - NLM
STAT- MEDLINE
DCOM- 20020627
LR  - 20161124
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 22
IP  - 2A
DP  - 2002 Mar-Apr
TI  - Monitoring for DNA damage of humans occupationally exposed to methyl bromide.
PG  - 997-1000
AB  - BACKGROUND: Methyl bromide (MeBr) is a methylating agent, weak mutagen and 
      possible animal carcinogen. A molecular epidemiological study to examine human 
      exposure to, and consequent DNA damage by MeBr was conducted in an area where 
      this agent is used extensively for soil sterilisation in greenhouses. MATERIALS 
      AND METHODS: During the first part of the study, blood samples were collected 
      from 21 persons within 24 hours after use of MeBr for greenhouse sterilisation, 
      as well as from 19 non-exposed subjects. Personal air sampling was also carried 
      out, indicating mean air concentrations for different subjects in the range 11-78 
      mg/m3. In the second part of the study, an attempt was made to examine 
      professional applicators of MeBr who suffered particularly high exposures (mean 
      exposures, based on personal monitoring 23-165 mg/m3). The levels of 
      N7-methylguanine and O6-methylguanine, two DNA adducts known to be induced by 
      MeBr, were assessed in blood leukocyte DNA. RESULTS: Concerning the first part, 
      two subjects (one exposed and one control) were found to be positive for 
      N7-methylguanine, while none of the blood samples analysed had detectable levels 
      of O6-methylguanine. Among 6 such persons examined during the second part, 2 were 
      found positive for N7-methylguanine while none was positive for O6-methylguanine. 
      CONCLUSION: Within the detection power of this limited study, no significant 
      evidence of induction of DNA damage in blood leukocyte DNA by MeBr was found.
FAU - Pletsa, V
AU  - Pletsa V
AD  - Laboratory of Chemical Carcinogenesis, Institute of Biological Research and 
      Biotechnology, National Hellenic Research Foundation, Athens, Greece. 
      vpletsa@eie.gr
FAU - Steenwinkel, M J S
AU  - Steenwinkel MJ
FAU - Stoikidou, M
AU  - Stoikidou M
FAU - van Delft, J H M
AU  - van Delft JH
FAU - Baan, R A
AU  - Baan RA
FAU - Katsouyanni, K
AU  - Katsouyanni K
FAU - Kyrtopoulos, S A
AU  - Kyrtopoulos SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA Adducts)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Mutagens)
RN  - 5Z93L87A1R (Guanine)
RN  - 661J4K04NB (7-methylguanine)
RN  - 9B710FV2AE (O-(6)-methylguanine)
RN  - 9V42E1Z7B6 (methyl bromide)
SB  - IM
MH  - Adult
MH  - Aged
MH  - DNA Adducts/blood
MH  - *DNA Damage
MH  - Female
MH  - Greece/epidemiology
MH  - Guanine/*analogs & derivatives/metabolism
MH  - Humans
MH  - Hydrocarbons, Brominated/*adverse effects
MH  - Leukocytes/drug effects/metabolism
MH  - Mutagens/*adverse effects
MH  - Occupational Exposure/*adverse effects
EDAT- 2002/05/17 10:00
MHDA- 2002/06/28 10:01
CRDT- 2002/05/17 10:00
PHST- 2002/05/17 10:00 [pubmed]
PHST- 2002/06/28 10:01 [medline]
PHST- 2002/05/17 10:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2002 Mar-Apr;22(2A):997-1000.