PMID- 12015086
OWN - NLM
STAT- MEDLINE
DCOM- 20020604
LR  - 20091119
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 23
IP  - 1
DP  - 2002 Jan
TI  - [Detection of inv (16) in acute myelomonocytic leukemia by interphase 
      fluorescence in situ hybridization].
PG  - 30-2
AB  - OBJECTIVE: To evaluate fluorescence in situ hybridization (FISH) in the detection 
      of inv (16) (p13; q22). METHODS: Spectrum red labeled yeast artificial chromosome 
      (YAC) clone 854E2 which spans the breakpoint cluster region in MYH11 in band 
      16p13 and single color interphase FISH were used to detect inv (16) in 26 cases 
      of acute myelomonocytic leukemias (AML-M(4)), and the results were compared with 
      that of conventional cytogenetic analysis. RESULTS: R banding karyotyping test 
      revealed no inv (16) in 25 cases, one AML M(4Eo) case showed inv (16) by G 
      banding. Nine cases including all three M(4Eo) had inv (16) by FISH analysis, 
      among whom the characteristic fluorescence signal pattern of the inv (16) was 
      seen in 13.3% to 32.1% (median, 21.3%) of the tested cells. CONCLUSION: YAC 854E2 
      and interphase FISH provide a powerful technique in the detection of inv (16) 
      (p13q22).
FAU - Li, Jianyong
AU  - Li J
AD  - First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, 
      Suzhou 215006, China.
FAU - Pan, Jinlan
AU  - Pan J
FAU - Wu, Yafang
AU  - Wu Y
FAU - Guo, Yu
AU  - Guo Y
FAU - Xue, Yongquan
AU  - Xue Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Inversion
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Leukemia, Myelomonocytic, Acute/*genetics/pathology
MH  - Male
MH  - Middle Aged
EDAT- 2002/05/17 10:00
MHDA- 2002/06/05 10:01
CRDT- 2002/05/17 10:00
PHST- 2002/05/17 10:00 [pubmed]
PHST- 2002/06/05 10:01 [medline]
PHST- 2002/05/17 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2002 Jan;23(1):30-2.