PMID- 12016028
OWN - NLM
STAT- MEDLINE
DCOM- 20020806
LR  - 20190922
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 772
IP  - 1
DP  - 2002 May 25
TI  - Determination of ciprofloxacin, enrofloxacin and flumequine in pig plasma samples 
      by capillary isotachophoresis--capillary zone electrophoresis.
PG  - 163-72
AB  - Quinolones are a group of synthetic antibiotics that are widely used in 
      veterinary medicine. Their residues may remain in tissues, milk, etc. intended 
      for human consumption. The European Union fixes the maximum residue limits (MRLs) 
      of veterinary medicinal products in foodstuffs of animal origin. Analytical 
      methods are therefore needed to determine them in biological samples. In this 
      study, we describe capillary isotachophoresis-capillary zone electrophoresis 
      (ITP-CZE) to analyze three quinolones, enrofloxacin (ENR), ciprofloxacin (CPR) 
      and flumequine (FLU), in pig plasma samples. We used solid-phase extraction with 
      Oasis HLB cartridges as a sample pretreatment clean-up step. Capillary zone 
      electrophoresis (CZE) requires low amounts of sample and is not as sensitive as 
      one would wish. ITP-CZE is an easy way to increase the sample loadability and 
      sensitivity. With this system sensitivity increases 40-fold. The detection limits 
      for CPR, ENR and FLU were 70, 85 and 50 microg l(-1), respectively, which were 
      lower than their MRLs in different kinds of samples. This method is simple and 
      sensitive, and is therefore an alternative tool to the existing HPLC methods for 
      analyzing the residuals of these quinolones in biological samples.
FAU - Hernandez, Margarita
AU  - Hernandez M
AD  - Departament de Quimica Analitica i Quimica Organica, Universitat Rovira i 
      Virgili, Placa Imperial Tarraco 1, 43005, Tarragona, Spain. socias@quimica.urv.es
FAU - Aguilar, Carme
AU  - Aguilar C
FAU - Borrull, Francesc
AU  - Borrull F
FAU - Calull, Marta
AU  - Calull M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Quinolizines)
RN  - 0 (Quinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - UVG8VSP2SJ (flumequine)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*blood
MH  - Ciprofloxacin/*blood
MH  - Electrophoresis, Capillary/*methods
MH  - Enrofloxacin
MH  - *Fluoroquinolones
MH  - Quinolizines/*blood
MH  - Quinolones/*blood
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2002/05/23 10:00
MHDA- 2002/08/07 10:01
CRDT- 2002/05/23 10:00
PHST- 2002/05/23 10:00 [pubmed]
PHST- 2002/08/07 10:01 [medline]
PHST- 2002/05/23 10:00 [entrez]
AID - S1570023202000715 [pii]
AID - 10.1016/s1570-0232(02)00071-5 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2002 May 25;772(1):163-72. doi: 
      10.1016/s1570-0232(02)00071-5.