PMID- 12016316
OWN - NLM
STAT- MEDLINE
DCOM- 20020607
LR  - 20231213
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Linking)
VI  - 296
IP  - 5571
DP  - 2002 May 17
TI  - Induction and suppression of RNA silencing by an animal virus.
PG  - 1319-21
AB  - RNA silencing is a sequence-specific RNA degradation mechanism that is 
      operational in plants and animals. Here, we show that flock house virus (FHV) is 
      both an initiator and a target of RNA silencing in Drosophila host cells and that 
      FHV infection requires suppression of RNA silencing by an FHV-encoded protein, 
      B2. These findings establish RNA silencing as an adaptive antiviral defense in 
      animal cells. B2 also inhibits RNA silencing in transgenic plants, providing 
      evidence for a conserved RNA silencing pathway in the plant and animal kingdoms.
FAU - Li, Hongwei
AU  - Li H
AD  - Department of Plant Pathology and Center for Plant Cell Biology, University of 
      California, Riverside, CA 92521, USA.
FAU - Li, Wan Xiang
AU  - Li WX
FAU - Ding, Shou Wei
AU  - Ding SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Luminescent Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA, Untranslated)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Agrobacterium tumefaciens/genetics
MH  - Animals
MH  - Cell Line
MH  - Drosophila/genetics/*virology
MH  - *Gene Silencing
MH  - Genes, Viral
MH  - Green Fluorescent Proteins
MH  - Luminescent Proteins/genetics
MH  - Nodaviridae/*genetics/*physiology
MH  - Plant Leaves/genetics/metabolism
MH  - Plants, Genetically Modified
MH  - RNA, Double-Stranded/genetics/metabolism
MH  - RNA, Small Interfering
MH  - RNA, Untranslated/*metabolism
MH  - RNA, Viral/genetics/metabolism
MH  - Nicotiana/*genetics/metabolism/microbiology
MH  - Transfection
MH  - Viral Proteins/genetics/*physiology
EDAT- 2002/05/23 10:00
MHDA- 2002/06/12 10:01
CRDT- 2002/05/23 10:00
PHST- 2002/05/23 10:00 [pubmed]
PHST- 2002/06/12 10:01 [medline]
PHST- 2002/05/23 10:00 [entrez]
AID - 296/5571/1319 [pii]
AID - 10.1126/science.1070948 [doi]
PST - ppublish
SO  - Science. 2002 May 17;296(5571):1319-21. doi: 10.1126/science.1070948.