PMID- 12019248
OWN - NLM
STAT- MEDLINE
DCOM- 20021220
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 161
IP  - 1
DP  - 2002 May
TI  - A molecular cytogenetic map of sorghum chromosome 1. Fluorescence in situ 
      hybridization analysis with mapped bacterial artificial chromosomes.
PG  - 345-53
AB  - We used structural genomic resources for Sorghum bicolor (L.) Moench to target 
      and develop multiple molecular cytogenetic probes that would provide extensive 
      coverage for a specific chromosome of sorghum. Bacterial artificial chromosome 
      (BAC) clones containing molecular markers mapped across sorghum linkage group A 
      were labeled as probes for fluorescence in situ hybridization (FISH). Signals 
      from single-, dual-, and multiprobe BAC-FISH to spreads of mitotic chromosomes 
      and pachytene bivalents were associated with the largest sorghum chromosome, 
      which bears the nucleolus organizing region (NOR). The order of individual 
      BAC-FISH loci along the chromosome was fully concordant to that of marker loci 
      along the linkage map. In addition, the order of several tightly linked molecular 
      markers was clarified by FISH analysis. The FISH results indicate that markers 
      from the linkage map positions 0.0-81.8 cM reside in the short arm of chromosome 
      1 whereas markers from 81.8-242.9 cM are located in the long arm of chromosome 1. 
      The centromere and NOR were located in a large heterochromatic region that spans 
      approximately 60% of chromosome 1. In contrast, this region represents only 0.7% 
      of the total genetic map distance of this chromosome. Variation in recombination 
      frequency among euchromatic chromosomal regions also was apparent. The integrated 
      data underscore the value of cytological data, because minor errors and 
      uncertainties in linkage maps can involve huge physical regions. The successful 
      development of multiprobe FISH cocktails suggests that it is feasible to develop 
      chromosome-specific "paints" from genomic resources rather than flow sorting or 
      microdissection and that when applied to pachytene chromatin, such cocktails 
      provide an especially powerful framework for mapping. Such a molecular 
      cytogenetic infrastructure would be inherently cross-linked with other genomic 
      tools and thereby establish a cytogenomics system with extensive utility in 
      development and application of genomic resources, cloning, transgene 
      localization, development of plant "chromonomics," germplasm introgression, and 
      marker-assisted breeding. In combination with previously reported work, the 
      results indicate that a sorghum cytogenomics system would be partially applicable 
      to other gramineous genera.
FAU - Islam-Faridi, M N
AU  - Islam-Faridi MN
AD  - Department of Soil and Crop Sciences, Texas A&M University, College Station, 
      Texas 77843, USA. nfaridi@neo.tamu.edu
FAU - Childs, K L
AU  - Childs KL
FAU - Klein, P E
AU  - Klein PE
FAU - Hodnett, G
AU  - Hodnett G
FAU - Menz, M A
AU  - Menz MA
FAU - Klein, R R
AU  - Klein RR
FAU - Rooney, W L
AU  - Rooney WL
FAU - Mullet, J E
AU  - Mullet JE
FAU - Stelly, D M
AU  - Stelly DM
FAU - Price, H J
AU  - Price HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Poaceae/*genetics
PMC - PMC1462096
EDAT- 2002/05/23 10:00
MHDA- 2002/12/21 04:00
PMCR- 2002/08/01
CRDT- 2002/05/23 10:00
PHST- 2002/05/23 10:00 [pubmed]
PHST- 2002/12/21 04:00 [medline]
PHST- 2002/05/23 10:00 [entrez]
PHST- 2002/08/01 00:00 [pmc-release]
AID - 10.1093/genetics/161.1.345 [doi]
PST - ppublish
SO  - Genetics. 2002 May;161(1):345-53. doi: 10.1093/genetics/161.1.345.