PMID- 12019265
OWN - NLM
STAT- MEDLINE
DCOM- 20020909
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 32
DP  - 2002 Aug 9
TI  - Identification of a novel transcriptional activator, BSAC, by a functional 
      cloning to inhibit tumor necrosis factor-induced cell death.
PG  - 28853-60
AB  - Tumor necrosis factor (TNF) is a multifunctional cytokine, which induces 
      proliferation or death in a cell type-dependent manner. We previously showed that 
      murine embryonic fibroblasts (MEFs) from TNF receptor-associated factor 2 (Traf2) 
      and Traf5 double-deficient (double knockout (DKO)) mice were highly susceptible 
      to TNF-induced cell death. By functional cloning to rescue DKO MEFs from 
      TNF-induced cell death, we have identified a novel gene, Bsac. BSAC is composed 
      of N-terminal basic, SAP (SAF-A/B, Acinus, PIAS), and coiled-coil domains. BSAC 
      is a nuclear protein, and overexpression of BSAC potently activates promoters 
      containing A + T-rich sequences named CArG boxes. Domain mapping analysis 
      revealed that both N-terminal basic and C-terminal proline-rich sequence are 
      required for the transcriptional activity. Overexpression of BSAC in DKO MEFs 
      partially inhibited TNF-induced cell death by suppressing activation of caspases. 
      Interestingly, inhibition of TNF-induced cell death was not observed in DKO MEFs 
      transfected with either N-terminal or C-terminal deletion mutant of BSAC, 
      revealing an intimate correlation between transcriptional activity and 
      antiapoptotic function. Recently, a human homologue of BSAC named MAL/MKL1 
      (megakaryocytic acute leukemia/megakaryoblastic leukemia-1) was identified as a 
      fusion transcript generated by t(1,22) translocation in acute megakaryoblastic 
      leukemia. Collectively, BSAC is a novel transcriptional activator with 
      antiapoptotic function, which may be involved in the leukemogenesis.
FAU - Sasazuki, Tomonari
AU  - Sasazuki T
AD  - Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, 
      Bunkyo-ku, Tokyo 113-8421, Japan.
FAU - Sawada, Taisuke
AU  - Sawada T
FAU - Sakon, Sachiko
AU  - Sakon S
FAU - Kitamura, Toshio
AU  - Kitamura T
FAU - Kishi, Takuma
AU  - Kishi T
FAU - Okazaki, Tatsuma
AU  - Okazaki T
FAU - Katano, Mitsuo
AU  - Katano M
FAU - Tanaka, Masao
AU  - Tanaka M
FAU - Watanabe, Mamoru
AU  - Watanabe M
FAU - Yagita, Hideo
AU  - Yagita H
FAU - Okumura, Ko
AU  - Okumura K
FAU - Nakano, Hiroyasu
AU  - Nakano H
LA  - eng
SI  - GENBANK/AF385582
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020517
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MRTFA protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Trans-Activators)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Northern
MH  - Cell Death
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins
MH  - Dose-Response Relationship, Drug
MH  - Fibroblasts/metabolism
MH  - Gene Library
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Leukemia/metabolism
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*biosynthesis/*chemistry
MH  - Oncogene Proteins, Fusion
MH  - Plasmids/metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - Tissue Distribution
MH  - Trans-Activators/*biosynthesis/*chemistry
MH  - *Transcription, Genetic
MH  - Transcriptional Activation
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2002/05/23 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/05/23 10:00
PHST- 2002/05/23 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/05/23 10:00 [entrez]
AID - S0021-9258(20)70290-5 [pii]
AID - 10.1074/jbc.M203190200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Aug 9;277(32):28853-60. doi: 10.1074/jbc.M203190200. Epub 2002 
      May 17.