PMID- 12020602
OWN - NLM
STAT- MEDLINE
DCOM- 20021114
LR  - 20190916
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 16
IP  - 3
DP  - 2002 Jun
TI  - Rat granulocyte colony-forming unit (CFU-G) assay for the assessment of 
      drug-induced hematotoxicity.
PG  - 281-8
AB  - To assess the drug-induced hematotoxicity to granulocyte progenitors, we 
      established a modified colony-forming assay using rat bone marrow cells (BMCs). 
      In the presence of various colony-stimulating factors (CSFs), rat BMCs were 
      disseminated on methylcellulose at a concentration of 1.3 x 10(4) cells/cm(2) (5 
      x 10(4) cells/0.5 ml/well in a 12-well plate). Mouse granulocyte-macrophage 
      colony-stimulating factor (mGM-CSF) stimulated the formation of almost all 
      macrophage colonies. Human granulocyte colony-stimulating factor (hG-CSF) alone 
      or in combination with mouse interleukin-3 (mIL-3) did not significantly effect 
      on the number of rat colony-forming units in culture (CFU-C). When BMCs were 
      seeded at 5.2 x 10(4) cells/cm(2) (5 x 10(5) cells/1 ml/dish in a 35-mm dish), 
      hG-CSF increased the number of the colonies in a dose-dependent manner, and 
      resulted in about 50 colonies at 50 ng/ml. The constituent cells of the colonies 
      were identified as neutrophils. Under these conditions, the effects of 
      5-fluorouracil (5-FU) on granulocyte colony-forming units (CFU-G) were examined 
      in rats and mice. The inhibitory effect of 5-FU on rat CFU-G was similar to the 
      effect on mouse CFU-G. These results indicate that the rat CFU-G induced by 
      hG-CSF is capable of being used for the evaluation of drug-induced 
      hematotoxicity.
FAU - Matsumura-Takeda, K
AU  - Matsumura-Takeda K
AD  - Tokushima Research Institute, Otsuka Pharmaceutical Co. Ltd, Tokushima, Japan. 
      kuniko_m_t@research.otsuka.co.jp
FAU - Kotosai, K
AU  - Kotosai K
FAU - Ozaki, A
AU  - Ozaki A
FAU - Hara, H
AU  - Hara H
FAU - Yamashita, S
AU  - Yamashita S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Drug Combinations)
RN  - 0 (Hemolysin Proteins)
RN  - 0 (Interleukin-3)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*drug effects/pathology
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay/*methods
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Fluorouracil/toxicity
MH  - Granulocyte Colony-Stimulating Factor/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hemolysin Proteins/*toxicity
MH  - Humans
MH  - Interleukin-3/pharmacology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/*drug effects/pathology
MH  - Toxicity Tests/*methods
EDAT- 2002/05/22 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/05/22 10:00
PHST- 2002/05/22 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/05/22 10:00 [entrez]
AID - S0278691502000480 [pii]
AID - 10.1016/s0278-6915(02)00048-0 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2002 Jun;16(3):281-8. doi: 10.1016/s0278-6915(02)00048-0.