PMID- 12020680
OWN - NLM
STAT- MEDLINE
DCOM- 20021008
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 442
IP  - 1-2
DP  - 2002 May 3
TI  - Effects of ginsenosides, active components of ginseng, on nicotinic acetylcholine 
      receptors expressed in Xenopus oocytes.
PG  - 37-45
AB  - We investigated the effects of ginsenosides, the active ingredient of ginseng, on 
      neuronal or muscle-type nicotinic acetylcholine receptor channel activity 
      expressed in Xenopus oocytes after injection of cRNA encoding bovine neuronal 
      alpha3beta4, alpha7 or human muscle alphabetadeltavarepsilon subunits. Treatment 
      with acetylcholine elicited an inward peak current (I(ACh)) in oocytes expressing 
      nicotinic acetylcholine receptor subtypes. Cotreatment with ginsenoside Rg2 and 
      acetylcholine inhibited I(ACh) in oocytes expressing with alpha3beta4 or 
      alphabetadeltavarepsilon but not in oocytes expressing alpha7 nicotinic 
      acetylcholine receptors. The inhibition of I(ACh) by ginsenoside Rg2 was 
      reversible and dose-dependent. The half-inhibitory concentrations (IC50) of 
      ginsenoside Rg2 were 60.2+/-14.1 and 15.7+/-3.5 microM in oocytes expressing 
      alpha3beta4 and alphabetadeltavarepsilon nicotinic acetylcholine receptors, 
      respectively. The inhibition of I(ACh) by ginsenoside Rg2 was voltage-independent 
      and noncompetitive. Other ginsenosides besides ginsenoside Rg2 also inhibited 
      I(ACh) in oocytes expressing alpha3beta4 or alphabetadeltavarepsilon nicotinic 
      acetylcholine receptors. The order of potency for the inhibition of I(ACh) was 
      ginsenoside Rg2>Rf>Re>Rg1>Rc>Rb2>Rb1 in oocytes expressing alpha3beta4 nicotinic 
      acetylcholine receptors and was ginsenoside Rg2>Rf>Rg1>Re>Rb1>Rc>Rb2 in oocytes 
      expressing alphabetadeltavarepsilon nicotinic acetylcholine receptors. These 
      results indicate that ginsenosides might regulate nicotinic acetylcholine 
      receptors in a differential manner and this regulation might be one of the 
      pharmacological actions of Panax ginseng.
FAU - Choi, Seok
AU  - Choi S
AD  - National Research Laboratory for the Study of Ginseng Signal Transduction and 
      Department of Physiology, College of Veterinary Medicine, Chonnam National 
      University, Kwangju, South Korea.
FAU - Jung, Se Yeon
AU  - Jung SY
FAU - Lee, Jun Ho
AU  - Lee JH
FAU - Sala, Francisco
AU  - Sala F
FAU - Criado, Manuel
AU  - Criado M
FAU - Mulet, Jose
AU  - Mulet J
FAU - Valor, Luis Miguel
AU  - Valor LM
FAU - Sala, Salvador
AU  - Sala S
FAU - Engel, Andrew G
AU  - Engel AG
FAU - Nah, Seung Yeol
AU  - Nah SY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Ginsenosides)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (Saponins)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Cattle
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gene Expression
MH  - Ginsenosides
MH  - Humans
MH  - Membrane Potentials/drug effects
MH  - Oocytes/drug effects/metabolism
MH  - Panax/*chemistry
MH  - Receptors, Nicotinic/genetics/*physiology
MH  - Saponins/chemistry/*pharmacology
MH  - Xenopus laevis
EDAT- 2002/05/22 10:00
MHDA- 2002/10/09 04:00
CRDT- 2002/05/22 10:00
PHST- 2002/05/22 10:00 [pubmed]
PHST- 2002/10/09 04:00 [medline]
PHST- 2002/05/22 10:00 [entrez]
AID - S001429990201508X [pii]
AID - 10.1016/s0014-2999(02)01508-x [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2002 May 3;442(1-2):37-45. doi: 10.1016/s0014-2999(02)01508-x.