PMID- 12020752
OWN - NLM
STAT- MEDLINE
DCOM- 20020722
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 71
IP  - 1
DP  - 2002 May 24
TI  - Transforming growth factor beta-1 attenuates endothelin-1-induced functions in 
      neonatal cardiac myocytes.
PG  - 99-113
AB  - In the present study we characterized a "crosstalk" mechanism between 
      transforming growth factor beta-1 (TGF beta-1) and endothelin-1 (ET1) signaling 
      pathways in neonatal cardiac myocytes. A 5 minute pretreatment with 1 ng/ml 
      concentrations of TGF beta-1 attenuated ET1-induced negative chronotropic effects 
      and translocation of the alpha, delta and varepsilonPKC isozymes to the 
      particulate cell fraction. We found no effect of TGF beta-1 on responses induced 
      by the P(2) purinergic agonist ATP or phorbol ester. Treatment of cardiac 
      myocytes with acidic fibroblast growth factor (aFGF) did not alter ET1- or 
      ATP-mediated effects on contraction rate or translocation of PKC isozymes to the 
      particulate fraction. Our studies suggest that TGF beta-1 may act as a negative 
      modulator of ET1- but not ATP- or phorbol ester-induced PKC isozyme signaling 
      events in neonatal cardiac myocytes. A better understanding of the complex ET1 
      and TGF beta-1 signaling mechanisms in neonatal heart cells should enhance our 
      knowledge regarding the interplay between these pathways.
FAU - Johnson, John A
AU  - Johnson JA
AD  - The Department of Pharmacology and Toxicology, School of Medicine, Institute of 
      Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 
      30912-2300, USA. jjohnson@mail.mcg.edu
FAU - Waller, Jennifer
AU  - Waller J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Endothelin-1)
RN  - 0 (Isoenzymes)
RN  - 0 (Phorbol Esters)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Animals, Newborn/*physiology
MH  - Autoradiography
MH  - Blotting, Western
MH  - Densitometry
MH  - Endothelin-1/*antagonists & inhibitors/pharmacology
MH  - Fibroblast Growth Factor 1/pharmacology
MH  - Heart/*drug effects
MH  - Heart Rate/drug effects
MH  - Isoenzymes/metabolism
MH  - Myocardial Contraction/drug effects
MH  - Myocardium/*cytology/enzymology
MH  - Phorbol Esters/pharmacology
MH  - Protein Kinase C/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor Cross-Talk
MH  - Receptors, Purinergic P2/drug effects
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Transforming Growth Factor beta1
EDAT- 2002/05/22 10:00
MHDA- 2002/07/23 10:01
CRDT- 2002/05/22 10:00
PHST- 2002/05/22 10:00 [pubmed]
PHST- 2002/07/23 10:01 [medline]
PHST- 2002/05/22 10:00 [entrez]
AID - S0024320502016247 [pii]
AID - 10.1016/s0024-3205(02)01624-7 [doi]
PST - ppublish
SO  - Life Sci. 2002 May 24;71(1):99-113. doi: 10.1016/s0024-3205(02)01624-7.