PMID- 12020803
OWN - NLM
STAT- MEDLINE
DCOM- 20020802
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1570
IP  - 3
DP  - 2002 Apr 15
TI  - Down-regulation of Id-1 expression is associated with TGF beta 1-induced growth 
      arrest in prostate epithelial cells.
PG  - 145-52
AB  - Transforming growth factor beta1 (TGF beta 1) plays important roles in the 
      regulation of cell growth and differentiation in both normal and malignant 
      prostate epithelial cells. Although certain pathways have been suggested, the 
      mechanisms responsible for the action of TGF beta 1 are not well understood. In 
      the present study, using a human papilloma virus 16 E6/E7 immortalized prostate 
      epithelial cell line, HPr-1, we report that TGF beta 1 was able to suppress the 
      expression of Id-1, a helix-loop-helix (HLH) protein, which plays important roles 
      in the inhibition of cell differentiation and growth arrest. In addition, a 
      decrease at both Id-1 mRNA and protein expression levels was associated with TGF 
      beta 1-induced growth arrest and differentiation, indicating that Id-1 may be 
      involved in TGF beta 1 signaling pathway. The fact that up-regulation of 
      p21(WAF1), one of the downstream effectors of Id-1, was observed after exposure 
      to TGF beta 1 further indicates the involvement of Id-1 in the TGF beta 1-induced 
      growth arrest in HPr-1 cells. However, increased expression of p27(KIP1) was also 
      observed in the TGF beta 1-treated cells, suggesting that in addition to 
      down-regulation of Id-1, other factors may be involved in the TGF beta 1-induced 
      cell growth arrest and differentiation in prostate epithelial cells. Our results 
      provide evidence for the first time that TGF beta 1 may be one of the upstream 
      regulators of Id-1.
FAU - Ling, M T
AU  - Ling MT
AD  - Department of Anatomy, Faculty of Medicine, 5/F, Li Shu Fan Building, 5 Sassoon 
      Road, University of Hong Kong, Hong Kong, SAR, China.
FAU - Wang, X
AU  - Wang X
FAU - Tsao, S W
AU  - Tsao SW
FAU - Wong, Y C
AU  - Wong YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ID1 protein, human)
RN  - 0 (Inhibitor of Differentiation Protein 1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Repressor Proteins)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
SB  - IM
MH  - Blotting, Western
MH  - Cell Cycle Proteins/biosynthesis
MH  - Cell Differentiation
MH  - Cell Division
MH  - Cell Line
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclin-Dependent Kinase Inhibitor p27
MH  - Cyclins/biosynthesis
MH  - DNA-Binding Proteins/*biosynthesis/physiology
MH  - *Down-Regulation
MH  - Epithelial Cells/metabolism
MH  - Flow Cytometry
MH  - Helix-Loop-Helix Motifs/*physiology
MH  - Humans
MH  - Inhibitor of Differentiation Protein 1
MH  - Male
MH  - Prostate/metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Transforming Growth Factor beta/metabolism
MH  - *Repressor Proteins
MH  - Transcription Factors/*biosynthesis/physiology
MH  - Transforming Growth Factor beta/*physiology
MH  - Transforming Growth Factor beta1
MH  - Tumor Suppressor Proteins/biosynthesis
MH  - Up-Regulation
EDAT- 2002/05/22 10:00
MHDA- 2002/08/03 10:01
CRDT- 2002/05/22 10:00
PHST- 2002/05/22 10:00 [pubmed]
PHST- 2002/08/03 10:01 [medline]
PHST- 2002/05/22 10:00 [entrez]
AID - S0304416502001897 [pii]
AID - 10.1016/s0304-4165(02)00189-7 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2002 Apr 15;1570(3):145-52. doi: 
      10.1016/s0304-4165(02)00189-7.