PMID- 12021309
OWN - NLM
STAT- MEDLINE
DCOM- 20020619
LR  - 20201226
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 195
IP  - 10
DP  - 2002 May 20
TI  - The CD8+ dendritic cell subset selectively endocytoses dying cells in culture and 
      in vivo.
PG  - 1289-302
AB  - Dendritic cells (DCs) are able in tissue culture to phagocytose and present 
      antigens derived from infected, malignant, and allogeneic cells. Here we show 
      directly that DCs in situ take up these types of cells after fluorescent labeling 
      with carboxyfluorescein succinimidyl ester (CFSE) and injection into mice. The 
      injected cells include syngeneic splenocytes and tumor cell lines, induced to 
      undergo apoptosis ex vivo by exposure to osmotic shock, and allogeneic B cells 
      killed by NK cells in situ. The CFSE-labeled cells in each case are actively 
      endocytosed by DCs in vivo, but only the CD8+ subset. After uptake, all of the 
      phagocytic CD8+ DCs can form major histocompatibility complex class II-peptide 
      complexes, as detected with a monoclonal antibody specific for these complexes. 
      The CD8+ DCs also selectively present cell-associated antigens to both CD4+ and 
      CD8+ T cells. Similar events take place with cultured DCs; CD8+ DCs again 
      selectively take up and present dying cells. In contrast, both CD8+ and CD8- DCs 
      phagocytose latex particles in culture, and both DC subsets present soluble 
      ovalbumin captured in vivo. Therefore CD8+ DCs are specialized to capture dying 
      cells, and this helps to explain their selective ability to cross present 
      cellular antigens to both CD4+ and CD8+ T cells.
FAU - Iyoda, Tomonori
AU  - Iyoda T
AD  - Department of Animal Development and Physiology, Graduate School of Biostudies. 
      Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 
      606-8502, Japan.
FAU - Shimoyama, Susumu
AU  - Shimoyama S
FAU - Liu, Kang
AU  - Liu K
FAU - Omatsu, Yoshiki
AU  - Omatsu Y
FAU - Akiyama, Yuji
AU  - Akiyama Y
FAU - Maeda, Yasuhiro
AU  - Maeda Y
FAU - Takahara, Kazuhiko
AU  - Takahara K
FAU - Steinman, Ralph M
AU  - Steinman RM
FAU - Inaba, Kayo
AU  - Inaba K
LA  - eng
GR  - P01 CA084512/CA/NCI NIH HHS/United States
GR  - R01 AI013013/AI/NIAID NIH HHS/United States
GR  - AI 13013/AI/NIAID NIH HHS/United States
GR  - CA 84512/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (CD8 Antigens)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - B-Lymphocytes/cytology/metabolism
MH  - CD8 Antigens/*metabolism
MH  - Cell Death
MH  - Dendritic Cells/*cytology/immunology/*metabolism
MH  - Flow Cytometry
MH  - Killer Cells, Natural/immunology
MH  - Liver/cytology
MH  - Lymph Nodes/cytology
MH  - Mice
MH  - Osmotic Pressure
MH  - *Phagocytosis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/cytology
MH  - Time Factors
MH  - Tumor Cells, Cultured
PMC - PMC2193756
EDAT- 2002/05/22 10:00
MHDA- 2002/06/20 10:01
PMCR- 2002/11/20
CRDT- 2002/05/22 10:00
PHST- 2002/05/22 10:00 [pubmed]
PHST- 2002/06/20 10:01 [medline]
PHST- 2002/05/22 10:00 [entrez]
PHST- 2002/11/20 00:00 [pmc-release]
AID - 020161 [pii]
AID - 10.1084/jem.20020161 [doi]
PST - ppublish
SO  - J Exp Med. 2002 May 20;195(10):1289-302. doi: 10.1084/jem.20020161.