PMID- 12027198
OWN - NLM
STAT- MEDLINE
DCOM- 20021118
LR  - 20190922
IS  - 0941-1291 (Print)
IS  - 0941-1291 (Linking)
VI  - 32
IP  - 4
DP  - 2002
TI  - Monocyte chemoattractant protein-1 expression in intimal hyperplasia of vein 
      grafts in a rat model.
PG  - 329-34
AB  - PURPOSE: Most cardiovascular surgeons prefer artery grafts to vein grafts. 
      Monocyte chemoattractant protein-1 (MCP-1) is the most potent chemoattractant 
      factor for monocytes/macrophages (Mo/Mphi). We conducted experiments based on our 
      hypothesis that intimal hyperplasia (IH) is more severe in vein grafts than in 
      situ artery grafts, due to differences in Mo/Mphi infiltration, and that the 
      expression of MCP-1 is different in vein grafts compared with in situ artery 
      grafts. METHODS: Lewis rats were subjected to either epigastric vein to common 
      femoral artery interposition grafts (VG: n = 34) or common femoral artery 
      reanastomoses (FA: n = 34). IH was defined as the intimal area/total area 
      (intimal ratio, Ri). Immunohistochemistry was also performed, using a monoclonal 
      antibody (ED-1), specific for rat Mo/Mphi. MCP-1 mRNA expression was examined by 
      a reverse transcription-polymerase chain reaction, and compared with S-26 mRNA 
      expression. RESULTS: After 2 weeks, the Ri of the VGs became significantly larger 
      than the Ri of the FA anastomoses (P < 0.01). ED-1+ cell numbers were 
      significantly elevated in the VG compared with the FA anastomoses, after 1 day, 2 
      weeks (P < 0.05), and 4 weeks (P < 0.01). MCP-1 mRNA expression was significantly 
      increased in the VGs by 1 day (P < 0.05). CONCLUSION: These results show that 
      there could be a direct correlation between the significant increases in MCP-1 
      mRNA expression, Mo/Mphi infiltration, and the development of IH in vein grafts, 
      not seen in FA anastomoses.
FAU - Mitsuhiro, Yamamura
AU  - Mitsuhiro Y
AD  - Department of Cardiovascular Surgery, Hyogo College of Medicine, Japan.
FAU - Miyamoto, Takashi
AU  - Miyamoto T
FAU - Hoch, John R
AU  - Hoch JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Surg Today
JT  - Surgery today
JID - 9204360
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Anastomosis, Surgical
MH  - Animals
MH  - Chemokine CCL2/*analysis/genetics
MH  - Femoral Artery/surgery
MH  - Hyperplasia
MH  - Immunohistochemistry
MH  - Male
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Tunica Intima/*metabolism/pathology
MH  - Vascular Patency
MH  - Veins/metabolism/*transplantation
EDAT- 2002/05/25 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/05/25 10:00
PHST- 2002/05/25 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/05/25 10:00 [entrez]
AID - 10.1007/s005950200047 [doi]
PST - ppublish
SO  - Surg Today. 2002;32(4):329-34. doi: 10.1007/s005950200047.