PMID- 12030365
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20220310
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 232
IP  - 1-2
DP  - 2002 Mar
TI  - Correlation between intrinsic mRNA stability and the affinity of AUF1 (hnRNP D) 
      and HuR for A+U-rich mRNAs.
PG  - 1-11
AB  - Presence of A+U-rich elements (AREs) within 3'-untranslated regions (3'UTRs) of 
      numerous mRNAs has been associated with rapid mRNA turnover; however, the 
      interaction of specific factors with AREs is also associated with mRNA 
      stabilization. Recently, two ARE binding proteins with putative mRNA 
      destabilizing (AUF1) and stabilizing (HuR) properties have been described. 
      However, no direct comparison ofAUF1 and HuR binding properties has been made. 
      Therefore, we examined the relative affinities of p37AUF1 and HuR for a diverse 
      set ofARE-containing mRNAs encoding beta-adrenergic receptors, a proto-oncogene, 
      and a cytokine. We find that high-affinity AUF1 binding appears to require 
      elements beyond primary nucleotide sequence. In contrast, binding of HuR appears 
      considerably less constrained. As a functional correlate, we determined the 
      ability of these specific mRNA sequences to affect the stability of chimeric 
      beta-globin mRNA constructs. Although the relative affinity ofAUF1 and HuR are 
      generally predictive of mRNA stability, we find that certain mRNA sequences do 
      not conform to these generalizations.
FAU - Blaxall, Burns C
AU  - Blaxall BC
AD  - Department of Pharmacology, University of Colorado Health Sciences Center, Denver 
      80262, USA.
FAU - Pende, Aldo
AU  - Pende A
FAU - Wu, Steven C
AU  - Wu SC
FAU - Port, J David
AU  - Port JD
LA  - eng
GR  - R01 HL051239/HL/NHLBI NIH HHS/United States
GR  - HL51239/HL/NHLBI NIH HHS/United States
GR  - GM07635/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Antigens, Surface)
RN  - 0 (ELAV Proteins)
RN  - 0 (ELAV-Like Protein 1)
RN  - 0 (ELAVL1 protein, human)
RN  - 0 (HNRNPD protein, human)
RN  - 0 (Heterogeneous Nuclear Ribonucleoprotein D0)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein D)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Regulatory Sequences, Ribonucleic Acid)
RN  - 56HH86ZVCT (Uracil)
RN  - 9004-22-2 (Globins)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - 3' Untranslated Regions/genetics/*metabolism
MH  - Adenine
MH  - Animals
MH  - *Antigens, Surface
MH  - Base Composition
MH  - Base Sequence
MH  - Cricetinae
MH  - ELAV Proteins
MH  - ELAV-Like Protein 1
MH  - Electrophoretic Mobility Shift Assay
MH  - Globins/*genetics
MH  - Half-Life
MH  - Heterogeneous Nuclear Ribonucleoprotein D0
MH  - Heterogeneous-Nuclear Ribonucleoprotein D/*metabolism
MH  - Humans
MH  - Protein Binding
MH  - Proto-Oncogene Mas
MH  - *RNA Stability
MH  - RNA-Binding Proteins/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Regulatory Sequences, Ribonucleic Acid/genetics
MH  - Time Factors
MH  - Transfection
MH  - Ultraviolet Rays
MH  - Uracil
EDAT- 2002/05/28 10:00
MHDA- 2003/02/07 04:00
CRDT- 2002/05/28 10:00
PHST- 2002/05/28 10:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2002/05/28 10:00 [entrez]
AID - 10.1023/a:1014819016552 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2002 Mar;232(1-2):1-11. doi: 10.1023/a:1014819016552.