PMID- 12030854 OWN - NLM STAT- MEDLINE DCOM- 20020718 LR - 20191106 IS - 1462-2912 (Print) IS - 1462-2912 (Linking) VI - 4 IP - 5 DP - 2002 May TI - Seasonal dynamics of particle-associated and free-living marine Proteobacteria in a salt marsh tidal creek as determined using fluorescence in situ hybridization. PG - 287-95 AB - The seasonal distributions of salt marsh free-living and particle-associated bacteria belonging to three subdivisions of the Proteobacteria were determined by fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). More than 66% (median = 78%) of total bacterial cells that were stainable with the fluorescent DNA stain Yo-Pro-1 were also detected using the bacterial probe EUB338. The alpha-Proteobacteria, especially those from the marine Rhodobacter group, were abundant on suspended particles and as free-living cells all year round. The marine Rhodobacter group constituted more than 25% of the particle-associated bacteria and more than 18% of the free-living bacteria. Probes specific for three subgroups within the marine Rhodobacter group detected more than 49% of the total marine Rhodobacter group cells. These subgroups displayed different seasonal dynamics. The marine Rhodobacter group is clearly a widespread, diverse and important bacterial lineage in bacterioplankton and particle-associated assemblages in south-eastern United States salt marshes at all times of the year. FAU - Dang, Hongyue AU - Dang H AD - Marine Science Program, University of South Carolina, Columbia, SC 29208, USA. FAU - Lovell, Charles R AU - Lovell CR LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - England TA - Environ Microbiol JT - Environmental microbiology JID - 100883692 SB - IM MH - Ecosystem MH - In Situ Hybridization, Fluorescence MH - Microscopy, Confocal MH - Proteobacteria/*growth & development MH - Seasons MH - South Carolina MH - *Water Microbiology EDAT- 2002/05/29 10:00 MHDA- 2002/07/19 10:01 CRDT- 2002/05/29 10:00 PHST- 2002/05/29 10:00 [pubmed] PHST- 2002/07/19 10:01 [medline] PHST- 2002/05/29 10:00 [entrez] AID - 295 [pii] AID - 10.1046/j.1462-2920.2002.00295.x [doi] PST - ppublish SO - Environ Microbiol. 2002 May;4(5):287-95. doi: 10.1046/j.1462-2920.2002.00295.x.