PMID- 12030925
OWN - NLM
STAT- MEDLINE
DCOM- 20020916
LR  - 20190916
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 33
IP  - 3
DP  - 2002 Jun
TI  - Characterization of the porcine sperm adhesion molecule gene SPAM1- expression 
      analysis, genomic structure, and chromosomal mapping.
PG  - 211-4
AB  - Sequence analysis of cDNA products, derived from adult porcine testis mRNA, gave 
      overlapping nucleotide sequence correlating to 1952 bp of the sperm adhesion 
      molecule 1 (SPAM1) gene. This sequence was shown to be homologous to SPAM1 genes 
      known in other mammalian species and contained an open reading frame encoding a 
      493-amino acid protein. Fluorescence in situ hybridization (FISH), using a 
      bacterial artificial chromosome (BAC) clone from the PigE BAC library, was used 
      to map SPAM1 to chromosome 18 of the pig. This finding is consistent with 
      comparative mapping experiments performed between pig and human chromosomes. 
      Polymerase chain reaction (PCR) analysis of genomic DNA has shown that the 1952 
      bp of cDNA sequence spans approximately 9 kb of genomic DNA and comprises of at 
      least four exons, with its size and structure being relatively conserved between 
      mouse, human and pig. Reverse transcriptase (RT)-PCR analysis of mRNA from nine 
      porcine tissues has also suggested that expression of SPAM1 is limited to the 
      testis.
FAU - Day, A E
AU  - Day AE
AD  - PIC International Group, Department of Pathology, University of Cambridge, 
      Cambridge, UK. aed21@cam.ac.uk
FAU - Quilter, C R
AU  - Quilter CR
FAU - Sargent, C A
AU  - Sargent CA
FAU - Mileham, A J
AU  - Mileham AJ
LA  - eng
SI  - GENBANK/AJ310675
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DNA, Complementary)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
RN  - EC 3.2.1.35 (hyaluronidase PH-20)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/*genetics
MH  - Chromosomes, Artificial, Bacterial
MH  - DNA, Complementary
MH  - Gene Expression
MH  - Hyaluronoglucosaminidase
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Physical Chromosome Mapping
MH  - Sequence Analysis, DNA
MH  - Swine/*genetics
EDAT- 2002/05/29 10:00
MHDA- 2002/09/17 10:01
CRDT- 2002/05/29 10:00
PHST- 2002/05/29 10:00 [pubmed]
PHST- 2002/09/17 10:01 [medline]
PHST- 2002/05/29 10:00 [entrez]
AID - 830 [pii]
AID - 10.1046/j.1365-2052.2002.00830.x [doi]
PST - ppublish
SO  - Anim Genet. 2002 Jun;33(3):211-4. doi: 10.1046/j.1365-2052.2002.00830.x.