PMID- 12031258
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 12
IP  - 11
DP  - 2001 Nov
TI  - Linoleic acid induces MCP-1 gene expression in human microvascular endothelial 
      cells through an oxidative mechanism.
PG  - 648-654
AB  - Linoleic acid is a dietary fatty acid that appears to play an important role in 
      activation of the vascular endothelium under a variety of pathological 
      conditions, including development of atherosclerosis or cancer metastasis. 
      Evidence indicates that inflammatory responses may be an underlying cause of 
      endothelial cell pathology induced by linoleic acid. However, the profile of 
      inflammatory mediators and the potential mechanisms involved in inflammatory 
      reactions stimulated by the exposure to linoleic acid are not fully understood. 
      The present study focused on the mechanisms of linoleic acid-induced expression 
      of monocyte chemoattractant protein-1 (MCP-1) gene in human microvascular 
      endothelial cells (HMEC-1). Treatment of HMEC-1 with increasing doses of linoleic 
      acid markedly activated an oxidative stress-responsive transcription factor, 
      nuclear factor-kappaB (NF-kappaB). In addition, exposure to linoleic acid induced 
      a time- and concentration-dependent overexpression of the MCP-1 gene. Increased 
      MCP-1 mRNA levels were observed in HMEC-1 treated with linoleic acid at doses as 
      low as 10 &mgr;M. Linoleic acid-induced overexpression of the MCP-1 gene was 
      associated with a significant elevation of MCP-1 protein levels. Most 
      importantly, preexposure of HMEC-1 to antioxidants, such as pyrrolidine 
      dithiocarbamate (PDTC) or N-acetylcysteine (NAC), attenuated linoleic 
      acid-induced MCP-1 mRNA expression. The obtained results indicate that linoleic 
      acid triggers MCP-1 gene expression in human microvascular endothelial cells 
      through oxidative stress/redox-related mechanisms.
FAU - Woo Lee, Yong
AU  - Woo Lee Y
AD  - Department of Surgery, Division of Neurosurgery, University of Kentucky Medical 
      Center, 800 Rose Street, 40536, Lexington, KY, USA
FAU - Joo Park, Hyen
AU  - Joo Park H
FAU - Hennig, Bernhard
AU  - Hennig B
FAU - Toborek, Michal
AU  - Toborek M
LA  - eng
GR  - P42 ES007380/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
EDAT- 2002/05/29 10:00
MHDA- 2002/05/29 10:01
CRDT- 2002/05/29 10:00
PHST- 2002/05/29 10:00 [pubmed]
PHST- 2002/05/29 10:01 [medline]
PHST- 2002/05/29 10:00 [entrez]
AID - S0955-2863(01)00186-3 [pii]
AID - 10.1016/s0955-2863(01)00186-3 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2001 Nov;12(11):648-654. doi: 10.1016/s0955-2863(01)00186-3.