PMID- 12034571
OWN - NLM
STAT- MEDLINE
DCOM- 20020703
LR  - 20031114
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 26
IP  - 6
DP  - 2002 Jun
TI  - Alveolar macrophage activation by myeloperoxidase: a model for exacerbation of 
      lung inflammation.
PG  - 716-22
AB  - Inflammation of the lung is characterized by the influx of increased numbers of 
      various leukocytes including polymorphonuclear leukocyte (PMN) neutrophils. In 
      addition to cells, numerous studies have pointed to the role of tumor necrosis 
      factor-alpha in the inflammatory process. This study addresses a previously 
      unrecognized interaction between neutrophil-derived myeloperoxidase (MPO) and 
      resident alveolar macrophages (AMo). Rat AMo exposed to either enzymatically 
      active recombinant MPO or enzymatically inactive MPO (iMPO) exhibited an 
      increased respiratory burst (RB). When iMPO was employed, the enhancement of the 
      RB was greater than that observed with MPO. Although the RB was greater with 
      iMPO, macrophage (Mo)-mediated intracellular candidic activity was equivalent for 
      both MPO and iMPO. It is known that pro- inflammatory cytokines contribute to the 
      inflammatory process. When rat AMo were exposed to both forms of myeloperoxidase, 
      iMPO demonstrated greater upregulation of cytokine genes as well as product. 
      These data suggest that at the site of inflammation, neutrophil-derived MPO and 
      iMPO stimulate AMo, resulting in an increased inflammatory and cytotoxic state, 
      and thereby contributing to the general lung inflammatory response.
FAU - Grattendick, Ken
AU  - Grattendick K
AD  - Department of Medical Microbiology and Immunology, University of South Florida, 
      College of Medicine, Tampa 33612-4799, USA. kgratten@hsc.usf.edu
FAU - Stuart, Rodney
AU  - Stuart R
FAU - Roberts, Erin
AU  - Roberts E
FAU - Lincoln, John
AU  - Lincoln J
FAU - Lefkowitz, Stanley S
AU  - Lefkowitz SS
FAU - Bollen, Alex
AU  - Bollen A
FAU - Moguilevsky, Nicole
AU  - Moguilevsky N
FAU - Friedman, Herman
AU  - Friedman H
FAU - Lefkowitz, Doris L
AU  - Lefkowitz DL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Cytokines)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Cytokines/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - *Macrophage Activation
MH  - Macrophages, Alveolar/*immunology
MH  - Mice
MH  - Peroxidase/*metabolism
MH  - Phagocytosis
MH  - Pneumonia/*immunology
MH  - Rats
MH  - Rats, Inbred Lew
EDAT- 2002/05/30 10:00
MHDA- 2002/07/04 10:01
CRDT- 2002/05/30 10:00
PHST- 2002/05/30 10:00 [pubmed]
PHST- 2002/07/04 10:01 [medline]
PHST- 2002/05/30 10:00 [entrez]
AID - 10.1165/ajrcmb.26.6.4723 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2002 Jun;26(6):716-22. doi: 10.1165/ajrcmb.26.6.4723.