PMID- 12037084
OWN - NLM
STAT- MEDLINE
DCOM- 20020813
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 6
DP  - 2002 Jun
TI  - Fluorescence in situ hybridization with peptide nucleic acid probes for rapid 
      identification of Candida albicans directly from blood culture bottles.
PG  - 2182-6
AB  - A new fluorescence in situ hybridization (FISH) method that uses peptide nucleic 
      acid (PNA) probes for identification of Candida albicans directly from 
      positive-blood-culture bottles in which yeast was observed by Gram staining 
      (herein referred to as yeast-positive blood culture bottles) is described. The 
      test (the C. albicans PNA FISH method) is based on a fluorescein-labeled PNA 
      probe that targets C. albicans 26S rRNA. The PNA probe is added to smears made 
      directly from the contents of the blood culture bottle and hybridized for 90 min 
      at 55 degrees C. Unhybridized PNA probe is removed by washing of the mixture (30 
      min), and the smears are examined by fluorescence microscopy. The specificity of 
      the method was confirmed with 23 reference strains representing phylogenetically 
      related yeast species and 148 clinical isolates covering the clinically most 
      significant yeast species, including C. albicans (n = 72), C. dubliniensis (n = 
      58), C. glabrata (n = 5), C. krusei (n = 2), C. parapsilosis (n = 4), and C. 
      tropicalis (n = 3). The performance of the C. albicans PNA FISH method as a 
      diagnostic test was evaluated with 33 routine and 25 simulated yeast-positive 
      blood culture bottles and showed 100% sensitivity and 100% specificity. It is 
      concluded that this 2.5-h method for the definitive identification of C. albicans 
      directly from yeast-positive blood culture bottles provides important information 
      for optimal antifungal therapy and patient management.
FAU - Rigby, Susan
AU  - Rigby S
AD  - Applied Biosystems, Bedford, Massachusetts 01730, USA.
FAU - Procop, Gary W
AU  - Procop GW
FAU - Haase, Gerhard
AU  - Haase G
FAU - Wilson, Deborah
AU  - Wilson D
FAU - Hall, Geraldine
AU  - Hall G
FAU - Kurtzman, Cletus
AU  - Kurtzman C
FAU - Oliveira, Kenneth
AU  - Oliveira K
FAU - Von Oy, Sabina
AU  - Von Oy S
FAU - Hyldig-Nielsen, Jens J
AU  - Hyldig-Nielsen JJ
FAU - Coull, James
AU  - Coull J
FAU - Stender, Henrik
AU  - Stender H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Culture Media)
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (RNA, Ribosomal, 23S)
SB  - IM
MH  - Blood/*microbiology
MH  - Candida albicans/*classification/genetics
MH  - Candidiasis/diagnosis/microbiology
MH  - Culture Media
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Probes/*genetics
MH  - Peptide Nucleic Acids/*genetics
MH  - RNA, Ribosomal, 23S/genetics
MH  - Sensitivity and Specificity
MH  - Time Factors
PMC - PMC130801
EDAT- 2002/05/31 10:00
MHDA- 2002/08/14 10:01
PMCR- 2002/06/01
CRDT- 2002/05/31 10:00
PHST- 2002/05/31 10:00 [pubmed]
PHST- 2002/08/14 10:01 [medline]
PHST- 2002/05/31 10:00 [entrez]
PHST- 2002/06/01 00:00 [pmc-release]
AID - 1582 [pii]
AID - 10.1128/JCM.40.6.2182-2186.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Jun;40(6):2182-6. doi: 10.1128/JCM.40.6.2182-2186.2002.