PMID- 12038623
OWN - NLM
STAT- MEDLINE
DCOM- 20020614
LR  - 20161124
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 20
IP  - 3
DP  - 2002 May
TI  - A novel bisphosphonate inhibits inflammatory bone resorption in a rat osteolysis 
      model with continuous infusion of polyethylene particles.
PG  - 499-505
AB  - This study examined the inhibitory effect of a new bisphosphonate (TRK-530) on 
      wear debris-mediated bone resorption in a rat osteolysis model involving 
      continuous infusion of high density polyethylene (HDPE) particles. TRK-530 (TRK) 
      is a novel synthetic bisphosphonate that has been shown to decrease the level of 
      tumor necrosis factor alpha (TNF-alpha) in the bone marrow of rats with adjuvant 
      arthritis. Forty Wistar rats were randomized to two groups (n = 20 each). In each 
      rat, a Kirshner (K) wire was inserted into the femur and HDPE particles were 
      continuously infused into the knee joint. Thereafter, the animals were 
      subcutaneously injected with saline (control group) or 1 mg/kg of TRK (TRK group) 
      every second day, and were sacrificed at 4 or 8 weeks after surgery. Radiographs 
      obtained at the time of sacrifice were evaluated for periprosthetic osteolysis. 
      We also examined the thickness of the reactive membrane as well as the number of 
      osteoclast-like cells around the K-wire. In addition, we examined the expression 
      of genes for bone-resorbing cytokines in the reactive membrane. Radiographic 
      peri-implant osteolysis was more frequent in the control group compared with the 
      TRK group at each time of assessment (p < 0.01). The interfacial membrane was 
      significantly thinner in the TRK group compared with the control group (p < 0.01) 
      and the average number of osteoclast-like cells around the K-wire was 
      significantly fewer in the TRK group (p < 0.01). In addition, the expression of 
      interleukin 1-alpha messenger ribonucleic acid (IL-1alpha mRNA) and TNF-alpha 
      mRNA was suppressed in the TRK group at each time of assessment. We conclude that 
      the TRK can inhibit the formation of inflammatory peri-implant osteolysis induced 
      by HDPE particles.
FAU - Iwase, Miho
AU  - Iwase M
AD  - Department of Orthopaedics, Tokyo Women's Medical University, Japan.
FAU - Kim, Kang Jung
AU  - Kim KJ
FAU - Kobayashi, Yoshiro
AU  - Kobayashi Y
FAU - Itoh, Masatoshi
AU  - Itoh M
FAU - Itoh, Tatsuo
AU  - Itoh T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Diphosphonates)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Arthritis/*drug therapy/etiology/pathology
MH  - Arthrography
MH  - Bone Resorption/*prevention & control
MH  - Diphosphonates/*therapeutic use
MH  - Female
MH  - Femur/diagnostic imaging/metabolism/pathology
MH  - Interleukin-1/genetics
MH  - *Knee Joint
MH  - Microspheres
MH  - Osteolysis/diagnostic imaging/*drug therapy/etiology
MH  - Polyethylene
MH  - Prostheses and Implants
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor-alpha/genetics
EDAT- 2002/06/01 10:00
MHDA- 2002/06/18 10:01
CRDT- 2002/06/01 10:00
PHST- 2002/06/01 10:00 [pubmed]
PHST- 2002/06/18 10:01 [medline]
PHST- 2002/06/01 10:00 [entrez]
AID - 10.1016/S0736-0266(01)00155-3 [doi]
PST - ppublish
SO  - J Orthop Res. 2002 May;20(3):499-505. doi: 10.1016/S0736-0266(01)00155-3.