PMID- 12041662
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20191025
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 75
IP  - 4
DP  - 2002 May
TI  - Mast cells derived from embryonic stem cells: a model system for studying the 
      effects of genetic manipulations on mast cell development, phenotype, and 
      function in vitro and in vivo.
PG  - 345-9
AB  - Large quantities of highly enriched populations of mast cells can be generated 
      from mouse embryonic stem (ES) cells using an in vitro differentiation system. 
      These embryonic stem cell-derived mast cells (ESMCs) exhibit many similarities to 
      mouse bone marrow-derived cultured mast cells (BMCMCs), including the abilities 
      to survive and to orchestrate immunologically specific immunoglobulin E 
      (IgE)-dependent reactions in vivo after transplantation into genetically mast 
      cell-deficient KitW/KitW-v mice. Coupled with the current spectrum of techniques 
      for genetically manipulating ES cells, ESMCs represent a unique model system to 
      analyze the effects of specific alterations in gene structure, expression, or 
      function, including embryonic lethal mutations, on mast cell development, 
      phenotype, and function in vitro and in vivo.
FAU - Tsai, Mindy
AU  - Tsai M
AD  - Department of Pathology, Stanford University School of Medicine, California 
      94305, USA.
FAU - Tam, See-Ying
AU  - Tam SY
FAU - Wedemeyer, Jochen
AU  - Wedemeyer J
FAU - Galli, Stephen J
AU  - Galli SJ
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Embryo, Mammalian/cytology
MH  - Mast Cells/*cytology
MH  - Methods
MH  - Mice
MH  - Models, Animal
MH  - Mutagenesis, Site-Directed
MH  - Recombination, Genetic
MH  - Stem Cells/*cytology
RF  - 21
EDAT- 2002/06/04 10:00
MHDA- 2003/07/12 05:00
CRDT- 2002/06/04 10:00
PHST- 2002/06/04 10:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2002/06/04 10:00 [entrez]
AID - 10.1007/BF02982122 [doi]
PST - ppublish
SO  - Int J Hematol. 2002 May;75(4):345-9. doi: 10.1007/BF02982122.